摘要
Background:To evaluate the accuracy and safety of micro radial and arcuate keratotomy incisions constructed by a femtosecond laser system with a curved contact patient interface in porcine eyes.Methods:Partial thickness micro radial and arcuate keratotomy incisions were constructed in porcine eyes with a femtosecond laser system and evaluated for precision of depth,quality,and consistency.Optical coherence tomography was used to determine the accuracy and precision of incision depth.Corneal endothelial safety was assessed by a fluorescent live/dead cell viability assay to demonstrate laser-induced endothelial cell loss.Quality was evaluated by ease of opening and examination of interfaces.Results:In two micro radial incision groups,intended incision depths of 50%and 80%resulted in mean achieved depths of 50.01%and 77.69%,respectively.In three arcuate incision groups,intended incision depths of 80%,600μm or 100μm residual uncut bed thickness resulted in mean achieved depths of 80.16%,603.03μm and residual bed of 115μm,respectively.No loss of endothelial cell density occurred when the residual corneal bed was maintained at a minimum of 85-116μm.The incisions were easy to open,and interfaces were smooth.Conclusions:A femtosecond laser system with curved contact interface created precise and reproducible micro radial and arcuate keratotomy incisions.Accuracy and precision of the incision depth and preservation of endothelial cell density demonstrated the effectiveness and safety of the system.