摘要
目的 使用高通量测序技术检测胃癌患者肠道菌群,探讨其分布及菌群功能的特点。方法 根据纳入排除标准,选取2019至2021年在河北北方学院附属第一医院胃肠肿瘤外科就诊的42例胃腺癌患者为研究对象,同时招募性别、年龄匹配的30例健康志愿者为对照组,收集两组粪便样本并提取DNA,使用16S核糖体DNA扩增子焦磷酸测序分析微生物种群,PICRUSt2预测菌群功能差异。结果 与健康对照组相比,胃癌组患者拟杆菌门丰度减少,变形菌门和放线菌门丰度增加,差异均有统计学意义(t=4.491、3.369、2.790,P<0.05);α多样性分析结果显示,胃癌患者Chao1指数升高而Shannon指数减少,差异均有统计学意义(t=10.884、9.953,P<0.01);主坐标分析(PCoA)显示,胃癌组与对照组菌群结构存在差异。线性判别效应分析(LefSe)结果显示,在属水平上,胃癌患者大肠埃希菌-志贺菌属(Escherichia-Shigella)丰度显著高于对照组,而作为益生菌的拟杆菌属(Bacteroides)、乳酸杆菌(Lactobacilli)显著低于对照组,差异均有统计学意义(线性判别分析值=3,P<0.05)。京都基因和基因组百科全书(KEGG)结果显示,胃癌患者与健康对照组间的菌群代谢存在差异。结论 胃癌患者肠道菌群多样性和结构均与健康人群不同,提示肠道菌群可能参与胃癌发生。
Objective By using high throughput sequencing to detect the intestinal flora of patients with gastric cancer, and to explore its distribution and the characteristics of flora function. Methods According to the inclusion and exclusion criteria, 42 patients with gastric adenocarcinoma admitted to the First Affiliated Hospital of Hebei North University from 2019 to 2021 were selected as the research subjects, and 30 gender-and age-matched healthy volunteers were recruited as the control group. Fecal samples were collected for extracting DNA in two groups. Microbial populations were analyzed using 16 S ribosomal DNA amplicon pyrosequencing. PICRUSt2(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) was used to identify functional differences of flora. Results Compared with control group, the abundance of Bacteroidetes decreased, while the abundance of Proteobacteria and Actinobacteria increased significantly in research group(t=4.491, 1.491, 3.369, P<0.05). The results of α diversity analysis showed that Chao1 index was increased, while Shannon diversity index was decreased in gastric cancer patients(t=10.884,9.953, P<0.01). Principal co-ordinates analysis(PCoA) showed the differences in the structure of flora between two groups. Linear discriminant analysis(LDA) effect size(LEfSe) analysis showed that the abundance of Escherichia-Shigella in gastric cancer patients was significantly higher than that in control group, while Bacteroides and Lactobacilli were significantly lower than those in control group(LDA=3, P<0.05). Kyoto Encyclopedia of Genes and Genomes(KEGG) showed the differences in gut microbiota metabolism between two groups. Conclusion The diversity and structure of intestinal flora in patients with gastric cancer are different from those in healthy people, suggesting that intestinal flora may be involved in the occurrence of gastric cancer.
作者
黄迪
李飞
苏卓彬
梁占东
孙光源
高建朝
李曙光
HUANG Di;LI Fei;SU Zhuo-bin;LIANG Zhan-dong;SUN Guang-yuan;GAO Jian-chao;LI Shu-guang(Department of Gastrointestinal Oncology Surgery,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)
出处
《中国临床研究》
CAS
2022年第3期303-306,共4页
Chinese Journal of Clinical Research
基金
河北省卫生厅科研基金项目(20180829)
张家口市科技计划项目(1521023D)。
关键词
胃癌
肠道菌群
高通量测序
埃希菌-志贺菌属
拟杆菌属
乳酸杆菌
Gastric cancer
Intestinal flora
High throughput sequencing
Escherichia-shigella
Bacteroides
Lactobacilli