大气压化学电离质谱法快速测定四种果蔬中阿维菌素农药残留

Determination of avermectin residues in four kinds of fruits and vegetables by LC-APCI-MS-MS

建立了一种蔬果中阿维菌素残留量的快速检测方法。参照改进的QuChERS方法处理后,使用大气压化学电离(atmospheric pressure chemical ionization,APCI)结合液相色谱-串联质谱法分析4种蔬果基质中的阿维菌素,统一考察检测方法的稳定性、基质效应、方法的准确度等指标。5 h内考察检测过程中标准溶液稳定性,每隔1 h对APCI模式下阿维菌素的标准溶液峰面积进行检测,方法的稳定性比值均在5%以内;结果表明,4种基质标准曲线的相关系数均大于0.99(线性范围为0.0125~0.40 mg/L);基质效应为0.97~1.05。在4种基质中添加0.01、0.1、0.2 mg/kg水平进行加标回收试验,添加回收率为93.3%~100.8%,相对标准偏差为1.8%~5.7%。该方法检测稳定性好,操作简便,基质影响小,准确度高,耗时短,适用于日常大批量水果和蔬菜中阿维菌素的定量测定。

A method for the determination of avermectin residues in fruits and vegetables was established in this study.Four matrices were treated with modified QuEChERS and liquid chromatography-tandem mass spectrometry(LC-MS-MS)with atmospheric pressure chemical ionization(APCI).The stability,matrix effect and method accuracy of the method was ed.The stability in 5 hours of the standard solution was below 5%tested every 1 hour.The results showed that correlation coefficients of standard curves in four matrices were greater than 0.99 in the linear range of 0.0125-0.40 mg/L;the matrix effect on the APCI mode was between 0.97 and 1.05.The spiked recovery results of avermectin in four matrices were 93.3%and 100.8%with the RSD of 1.8%to 5.7%(when in the adding amount of 0.01,0.1 and 0.2 mg/kg).The proposed method was more suitable for the quantitative analysis of avermectin in agricultural products for its stability,convenience,accuracy and less matrix effect.