农家品种‘白大头’及其衍生系‘天00127’苗期抗条锈性遗传分析

Inheritance of resistance to Puccinia striiformis f.sp.tritici in wheat landrace‘Baidatou’and its derivative‘Tian 00127’at seedling stage

2019年在甘肃省农业科学院植物保护研究所兰州温室,对抗条锈性优异的农家品种‘白大头’及其衍生系‘天00127’与感病品种‘铭贤169’杂交组合的P_(1)、P_(2)、F_(1)、F_(2)和BC_(1)代材料,苗期分别人工接种条锈菌主要流行小种CYR34、CYR32的单孢菌系,进行抗病性遗传分析,结果表明:供试亲本‘白大头’和‘天00127’对CYR34和CYR32均表现免疫。各世代材料中,‘铭贤169’/‘白大头’组合与‘铭贤169’/‘天00127’组合的F_(1)代对供试小种均表现免疫或近免疫;F_(2)代植株表现抗感分离,其中‘铭贤169’/‘白大头’组合对CYR34符合1R∶3S的理论比值,对CYR32符合3R∶1S的理论比值;BC_(1)代植株对CYR34表现全感,对CYR32符合1R∶1S的理论比值;‘铭贤169’/‘天00127’组合对CYR34和CYR32均符合3R∶1S的理论比值;BC_(1)代植株抗感分离符合1R∶1S的理论比值。表明供试材料‘白大头’对CYR34和CYR32的抗性分别由1对隐性和1对显性抗性基因控制,‘天00127’对CYR34、CYR32的苗期抗性均由1对显性抗性基因控制。推测‘天00127’对CYR32的抗病基因可能来源于亲本农家品种‘白大头’或‘85-173-4’。

The seedling resistance of wheat landrace‘Baidatou’and its derivative‘Tian 00127’by crossing with the susceptible variety‘Mingxian 169’and their offspring plants including F_(1),F_(2),and BC_(1) to CYR34,CYR32 of Puccinia striiformis f.sp.tritici(Pst)were evaluated in the Lanzhou greenhouse,Institute of Plant Protection,Gansu Academy of Agricultural Sciences(IPP,GAAS)in 2019.The results showed that the landrace‘Baidatou’and its derivative‘Tian 00127’were immune to CYR34 and CRY32.The F_(1) plants derived from the cross of‘Mingxian 169’with‘Baidatou’and the cross of‘Mingxian 169’with‘Tian 00127’were both immune or closely immune to CYR34 and CYR32.The segregation ratio for the cross between‘Mingxian 169’and‘Baidatou’accorded with the expected ratio of 1R∶3S to CYR34,and 3R∶1S to CYR32 in F_(2) plants;and all BC_(1) plants were susceptible to CYR34,but with a ratio of 1R∶1S to CYR32.The segregation ratio for the cross between‘Mingxian 169’and‘Tian 00127’accorded with the expected ratio of 3R∶1S to CYR34 and CYR32 in F_(2) plants,and BC_(1) plants showed an expected segregation ratio 1R∶1S.The results indicated that the resistance of wheat landrace‘Baidatou’was controlled by one recessive gene to CYR34,by one dominant gene to CYR32.The resistance of wheat‘Tian 00127’was controlled by one dominant gene to CYR34 and CYR32.The resistance of wheat variety‘Tian 00127’to CYR 32 might originate from the parents‘Baidatou’or‘85-173-4’.