甘肃省部分地区马铃薯晚疫病菌交配型及线粒体DNA单倍型分析

Analysis of mating type and mitochondrial DNA haplotypes of Phytophthora infestans collected from part areas in Gansu province

由致病疫霉Phytophthora infestans(Mont.)de Bary侵染引起的马铃薯晚疫病是一种世界范围内的毁灭性病害。为了明确甘肃省马铃薯晚疫病菌的遗传结构,对2018年甘肃省定西市、张掖市和陇南市采集的130株晚疫病菌进行了交配型和线粒体DNA(mtDNA)单倍型测定。结果表明:从130株晚疫病菌中检测到A1和A2两种交配型,A1交配型为14株(10.77%),A2交配型为116株(89.23%);检测到Ⅰa、Ⅱa和Ⅱb 3种线粒体DNA单倍型,发生频率分别为88.46%、3.85%和7.69%。Ⅰa单倍型菌株的交配型为A1和A2,Ⅱa单倍型菌株交配型为A1和A2,Ⅱb单倍型菌株交配型为A1。该结果表明甘肃省部分地区马铃薯晚疫病菌以A2交配型,Ⅰa mtDNA单倍型为主。甘肃省各采集地晚疫病菌群体结构或与当地种薯繁育、调运有关。

Potato late blight caused by Phytophthora infestans(Mont.)de Bary is a destructive disease in the world.In order to clarify the genetic structure of P.infestans in Gansu province,the mating type and mtDNA haplotype of 130 P.infestans strains collected in Dingxi city,Zhangye city and Longnan city in 2018 were determined.The results showed that A1 and A2 mating types were detected from 130 strains,among which 14 strains(10.77%)were the A1 mating type and 116 strains(89.23%)were A2.Three mtDNA haplotypes ofⅠa,Ⅱa andⅡb were detected from 130 strains,and the occurrence frequencies were 88.46%,3.85%and 7.69%,respectively.The mating types ofⅠa haplotype strains were A1 and A2,Ⅱa haplotype strains were A1 and A2,andⅡb haplotype strains were A1.The results showed that the main pathogens of P.infestans in Gansu province were A2 mating type andⅠa mtDNA haplotype.The population structure of P.infestans in different collection sites in Gansu province may be related to the breeding and transportation of local seed potatoes.