甘草查尔酮A可能通过调控miR-506-5p对宫颈癌细胞增殖和凋亡的分子机制

Licochalcone A may regulate the molecular mechanism of miR-506-5p on the proliferation and apoptosis of cervical cancer cells

目的探讨甘草查尔酮A(licochalconeA,Lic A)可能通过调控miR-506-5p对宫颈癌细胞增殖和凋亡的分子机制。方法体外培养宫颈癌细胞系SiHa,将细胞分为Control组、Lic A 15μmol/L组、Lic A 30μmol/L组、Lic A45μmol/L组、Lic A+anti-miR-NC组、Lic A+anti-miR-506-5p组。然后用MTT检测细胞增殖变化;采用流式细胞术检测细胞周期、凋亡情况;蛋白免疫印迹(Westernblot)检测CyclinD1、CDK4、Bcl-2、Bax蛋白表达;实时荧光定量PCR检测miR-506-5p表达变化。结果与Control组比较,Lic A 15μmol/L组、Lic A 30μmol/L组、Lic A 45μmol/L组细胞活性、S期细胞比例、CyclinD1、CDK4、Bcl-2蛋白表达明显降低,G0/G1期细胞比例、凋亡率、Bax蛋白表达、miR-506-5p表达水平显著增加。与Lic A+anti-miR-NC组相比,Lic A+anti-miR-506-5p组miR-506-5p表达水平、G0/G1期细胞比例、凋亡率、Bax蛋白表达明显降低,细胞活性、S期细胞比例、CyclinD1、CDK4、Bcl-2蛋白表达明显增加。结论甘草查尔酮A可能通过调控miR-506-5p抑制宫颈癌细胞增殖和阻滞细胞周期,并促进细胞凋亡。

Objective To investigate the molecular mechanism that licochalcone A(Lic A) may regulate the proliferation and apoptosis of cervical cancer cells by regulating miR-506-5 p. Methods The cervical cancer cell line SiHa was cultured in vitro,and the cells were divided into Control group Lic A 15 μmol/L group, Lic A 30 μmol/L group, Lic A 45 μmol/L group, Lic A+anti-miR-NC group, Lic A+anti-miR-506-5 p group. MTT to detect cell proliferation changes;flow cytometry to detect cell cycle and apoptosis;Western blot to detect CyclinD1, CDK4, Bcl-2, Bax protein expression;real-time fluorescent quantitative PCR to detect miR-506-5 p express changes. Results Compared with the control group, the cell viability, S phase cell ratio, CyclinD1, CDK4, and Bcl-2 protein expression in the Lic A 15 μmol/L group, Lic A 30 μmol/L group, and Lic A 45 μmol/L group were significantly reduced, and G0/G1 phase cell ratio, apoptosis rate, Bax protein expression, miR-506-5 p expression level increased significantly. Compared with the Lic A+anti-miR-NC group, the miR-506-5 p expression level, G0/G1 phase cell ratio,apoptosis rate, and Bax protein expression in the Lic A+anti-miR-506-5 p group were significantly reduced, cell viability, S phase cell ratio, CyclinD1, CDK4, and Bcl-2 protein expression increased significantly. Conclusion Licochalcone A may inhibit the proliferation of cervical cancer cells and block the cell cycle by regulating miR-506-5 p, and promote cell apoptosis.