不同PTH、PTHrP多肽对MDA-MB-231乳腺癌细胞系增殖的影响

Effects of different PTH and PTHrP polypeptides on the proliferation of MDA-MB-231 human breast carcinoma cell line

目的探究甲状旁腺激素(PTH)1-34、甲状旁腺激素相关蛋白(PTHrP)1-33、PTHrP 1-36、PTHrP 1-86多肽对乳腺癌细胞系增殖的影响。方法使用多肽对乳腺癌细胞系MDA-MB-231进行处理。结果培养条件为2%FBS、处理时间为24 h时,PTH 1-34、PTHrP 1-33、PTHrP 1-36、PTHrP 1-86都对细胞增殖有显著抑制作用(P<0.001);加入PTHrP 1-86多肽处理48 h时,10 nmol/L、25 nmol/L处理组别对细胞增殖有促进作用(P<0.05)。结论本实验证明PTH 1-34、PTHrP 1-33、PTHrP 1-36、PTHrP 1-86皆对乳腺癌细胞系MDA-MB-231的生长有调控作用,可能参与细胞增殖的信号通路。长远来看,本研究有助于开发通过抑制乳腺癌细胞增殖进行癌症治疗的新疗法。

Objective To analyze the effects of parathyroid hormone(PTH) 1-34, parathyroid hormone-related protein(PTHrP) 1-33, PTHrP 1-36, and PTHrP 1-86 peptides on the proliferation of human breast carcinoma cell line. Methods The human breast carcinoma cell line MDA-MB-231 were treated by peptides. Results PTH 1-34、PTHrP 1-33、PTHrP 1-36、PTHrP 1-86 inhibit cells proliferation significantly cultured in 2%FBS for 24 h(P<0.001). After treated for 48 h with 10 nmol/L, 25 nmol/L PTHrP 1-86, cells proliferation was significantly inhibited(P<0.05). Conclusion PTH 1-34, PTHrP 1-33,PTHrP 1-36, PTHrP 1-86 have regulatory effects on MDA-MB-231 cells proliferation. The peptides maybe involve in the signal pathway of cell proliferation. In the long run, this research could help develop new therapies for cancer by inhibiting the proliferation of breast cancer cells.