摘要
为了明确哈密某牛场犊牛死亡的病因,对菌株进行了分离纯化、革兰氏染色镜检、16S rDNA测序鉴定、小鼠致病性试验、药敏试验检测。结果表明,该分离菌为肺炎克雷伯氏菌,对小鼠致病性强。该分离菌株对硫酸阿米卡星、左氧氟沙星敏感;对阿奇霉素、头孢曲松钠、替米考星注射液和乳酸环丙沙星中度敏感;对氟苯尼考、头孢拉定、盐酸林可霉素、克林霉素、硫酸庆大霉素耐药。
In order to clarify the death cause of calves in a cattle farm in Hami, the strain was isolated and purified,examined by Gram staining microscopy, 16S rDNA sequencing test, mouse pathogenicity test, and drug susceptibility test.The results showed that the isolate was Klebsiella pneumoniae, which was highly pathogenic to mice. The bacterial was sensitive to amikacin sulfate and levofloxacin, it was moderately sensitive to azithromycin, ceftriaxone sodium, tilmicosin injection and ciprofloxacin lactate, it was resistant to florfenicol, cefradine, lincomycin hydrochloride, clindamycin and gentamicin sulfate.
作者
毋状元
仲荣
李璋赟
李威
金映红
汪萍
薛晶
陈古丽
郭会玲
阿依江·木合塔尔
夏俊
WU Zhuangyuan;ZHONG Rong;LI Zhangyun;LI Wei;JIN Yinghong;WANG Ping;XUE Jing;CHEN Guli;GUO Huiling;Ayijiang Muhetar;XIA Jun(Altay Animal Husbandry Workstation,Altay Xinjiang 836500;College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832003;Center for Animal Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi Xinjiang 830000;Institute of Veterinary Medicine,Xinjiang Academy of Animal Husbandry(Animal Clinical Medical Research Center,Xinjiang Academy of Animal Husbandry),Urumqi Xinjiang 830000)
出处
《现代农业科技》
2022年第6期150-152,156,共4页
Modern Agricultural Science and Technology
基金
肉牛高效健康饲养技术集成研究与示范(2021A2003-1)
自治区奶产业技术体系“奶牛常见病防控技术研究及应用”
自治区动物疫情监测与防治项目“牛羊常见多发疫病防治技术指导及绵羊传染性胸膜肺炎诊断试剂盒推广应用”。
关键词
牛
肺炎克雷伯氏菌
分离鉴定
药敏试验
cattle
Klebsiella pneumoniae
isolation and identification
drug sensitivity test