油田污染土壤土著菌的高盐高油驯化液理化特性研究

Physicochemical Properties of Culture Medium for Oilfield Indigenous Bacteria Domestication under High Oil/Salt Stress Conditions

为了获得高盐、高油优势菌株,对新疆白碱滩油田老化污染土壤中的土著菌进行了人为筛选驯化,考察了培养菌液理化参数的变化规律。结果表明:浊度、光密度不断增大,预示短期(72 h)内成功驯化出了一定数量的适应高盐(70000 mg/L)、高油(8100 mg/L)胁迫环境的菌株;pH由7.6逐渐降为5.9,这是因为耐盐菌降解石油烃过程中生成了羧酸、CO_(2)等酸性物质;受pH降低趋势的影响,氧化还原电位逐渐增大;单个驯化阶段内,耐盐菌逐渐调整渗透压进而适应高盐高油的胁迫条件。

In order to obtain the dominant strain with high salt and oil content,the indigenous bacteria from aging contaminated soil of Xinjiang Baijiantan oilfield was artificially acclimated,and the changes of physicochemical parameters of culture medium were investigated.As is shown in the results,the increasing turbidity and optical density indicated that a certain number of strains adapted to high salt(70000 mg/L)and high oil(8100 mg/L)stress were successfully domesticated in a short period(72 h);pH decreased from 7.6 to 5.9,because petroleum hydrocarbon degradation may produce carboxylic acid,CO_(2) and other acidic substances;under the influence of pH decreasing trend,oxidation-reduction potential increased gradually;in a single acclimation stage,halophilic bacteria gradually adjusted osmotic pressure to adapt to higher salt and oil stress conditions.