低共熔溶剂修饰分子印迹聚合物分离刺五加中的刺五加苷B、刺五加苷E、异嗪皮啶

Molecularly Imprinted Polymer Modified by Deep Eutectic Solvent for the Separation of Syringin,Eleutheroside E and Isofraxidin from Acanthopanax senticosus

以刺五加的根茎为原料,采用低共熔溶剂协同微波磁场进行萃取并对其萃取机理进行阐释。并合成了三分子模板印迹聚合物,实现对提取液中刺五加苷B、苷E、异嗪皮啶的同时吸附分离。结果表明:单因素试验得出的最优萃取条件为以氯化胆碱-乙二醇(n(氯化胆碱)∶n(乙二醇)=1∶3,V(氯化胆碱-乙二醇)∶V(H_(2)O)=5∶5))配制的低共熔溶剂水溶液为提取溶剂,提取料液比为m(刺五加根茎粉末)∶V(低共熔溶剂水溶液)=1 g∶10 mL,微波时间10 min,微波功率400 W。刺五加苷B、苷E、异嗪皮啶的提取率分别为0.282、1.486、0.0485 mg·g^(-1)。制备同时富集三者的固相萃取柱,优化固相萃取过程,得出淋洗溶剂为1.0 mL的水,模板分子的损失率分别为10.8%、17.1%、6.9%,洗脱溶剂为2.0 mL V(甲醇)∶V(乙酸)=95∶5的甲醇-乙酸溶液(此时淋洗溶剂为0.25 mL H_(2)O),洗脱后刺五加苷B、苷E、异嗪皮啶的回收率分别为92.8%、94.3%、88.4%。

We used the deep eutectic solvent and microwave magnetic field in extraction from the Acanthopanax senticosus,and explained the microwave extraction mechanism.The three-molecule template imprinted polymer was prepared to adsorb and separate Syringin,Eleutheroside E and Isofraxidin in the extractsimultaneously.A single factor research was conducted and the result showed:the optimum conditions were that choosing deep eutectic solvent as choline chloride-ethylene glycol(1∶3),and the volume fraction 50%DES solution as the extraction solvent,extraction solid-liquid ratio of 1 g∶10 mL,microwave time 10 min,microwave power 400 W,the contents of syringin,eleutheroside E and isofraxidin were 0.282,1.486,and 0.0485 mg/g,respectively.Prepared a solid-phase extraction column that simultaneously enriches the three,and the solid-phase extraction process was optimized.It was concluded that 1.0 mL H_(2)O was used as the washing solvent,the loss rates of the template molecule were 10.8%,17.1%,6.90%,and 2.0 mL MeOH-HOAc was used as the eluant(the washing solvent was 0.25 mL H_(2)O at this time),V(MeOH)∶V(HOAc)=95∶5.The recovery rates of syringin,eleutheroside E and isofraxidin were 92.8%,94.3%,88.4%,respectively.