小蓟内生真菌Chaetomium Bostrychodes次级代谢产物及其生物活性研究

Secondary Metabolites and Their Biological Activities of Chaetomium Bostrychodes fromCirsium Setosum

目的:对一株小蓟内生旋丝毛壳菌(Chaetomium bostrychodes)次级代谢产物化学结构及生物活性进行研究。方法:利用柱层析、Sephadex-20、HPLC等方法对发酵产物进行分离纯化,经核磁共振波谱、高分辨质谱技术并结合参考文献确定化合物结构,采用二倍稀释法检测化合物的抗菌活性。结果:从上述发酵产物中分离得到9个单体化合物,分别为aurasperone A(1)、fonsecinone A(2)、granulolactone(3)、4-hydroxyphenylacetone(4)、latifolicinin C(5)、p-hydroxybenzoic acid ethylester(6)、p-hydroxybenzoic acid(7)、phallac acid A(8)、1,2,4-benzenetriol(9),以上化合物均为首次从该菌种中分离得到。对这些化合物的抗临床耐药细菌和植物病源真菌活性进行测试,结果显示均无显著抑制作用。

Objective:The secondary metabolites and their biological activities of Chaetomium bostrychodes,an endophytic fungui isolated fromCirsium setosum,are investigated.Methods:The solid culture was separated and purified by column chromatography,Sephadex-20 and HPLC.The compounds were identified by NMR spectra,HRESIMS data and reference materials.The antibacterial and antifungal activity of the compounds was detected by two fold dilution method.Results:Nine compounds were isolated from the solid culture of C.bostrychodes.Their structures were identified as aurasperone A(1)、fonsecinone A(2)、granulolactone(3)、4-hydroxyphenylacetone(4)、latifolicinin C(5)、p-hydroxybenzoic acid ethylester(6)、p-hydroxybenz-oic acid(7)、phallac acids A(8)、1,2,4-benzenetriol(9)by the NMR spectra and HRESIMS data.All the above compounds were isolated from this strain for the first time and the biological activities of these compounds against clinical drug-resistant bacteria and plant pathogenic fungi were tested.However,none of them showed significant inhibitory activity.