不同提取方法对白花前胡指标成分含量的影响

Effect of Index Components in Peucedanum Praerupterum by Different Extraction Methods

目的:考察不同提取方式对前胡药材中白花前胡甲素和白花前胡乙素的影响,探寻白花前胡指标成分提取方法。方法:采用超声法和回流法提取,以HPLC法测定指标成分含量。结果:4种不同土壤类型的白花前胡样品的甲素和乙素含量存在差异;回流法制样处理白花前胡甲素含量均高于超声法制样,而乙素含量则相反。结论:回流法有利于白花前胡甲素的提取,较超声法提高了8.7%~21.9%;超声法有利于白花前胡乙素的提取,较回流法提高了10.4%~21.95%。选择合适的提取体系和方式可提高白花前胡有效成分的可检测性。

Objective:In order to explore the extraction method of the index components of Peucedanum praerupterum,the effects of different extraction methods on praeruptorum A and B were investigated.Methods:Determination of index components in Peucedanum praerupterum by ultrasonic and reflux methods using High-Performance Liquid Chromatography.Results:There are differences in the content of praeruptorin A and B among Peucedanum praerupterum grown in different soil types.The content of praeruptorin A in the reflux method was higher than that in the ultrasonic method,while the content of praeruptorin B was the opposite.Conclusion:The reflux method is favorable for the extraction of praeruptorin A,which is 8.7%~21.9% higher than the ultrasonic method;the ultrasonic method is beneficial for the extraction of praeruptorin B,which is 10.4%~21.95% higher than the reflux method.Selecting the appropriate extraction system and method can improve the detectability of the index components of Peucedanum praerupterum.