大豆ERF家族基因生物信息学分析及其对低磷胁迫的响应

Bioinformatics Analysis on Soybean ERF Family Genes and Their Response to Low Phosphorus Stress

为分析大豆乙烯响应因子(Ethylene Response Factor,ERF)基因家族的特征及其在低磷胁迫下的响应作用,本研究首先利用生物信息学方法从大豆基因组数据库中鉴定获得ERF家族基因,进行进化分析、染色体分布和基因结构分析,并研究其在大豆发育过程中不同组织的表达模式及在低磷胁迫下的表达情况。结果显示:59个大豆ERF家族基因分布在除4号染色体外的整个大豆染色体组上,其中3号、10号、13号、18号和19号染色体分布较多。基因结构分析显示整个家族具有典型的AP2结构域。同源序列比对和进化分析将该家族的基因分为4组,分别包括15,5,19和20个基因。基于Soybase数据库的组织表达分析表明该家族基因在大豆的不同组织中均有表达;qRT-PCR结果表明该家族基因低磷胁迫条件下的大豆根、茎和叶中均被诱导表达。结果说明大豆ERF基因结构较保守,能够参与低磷胁迫响应。

In order to analyze the characteristics of soybean Ethylene Response Factor(ERF)genes and their response to low phosphorus stress,this study identified ERF family genes from soybean genome database by bioinformatics methods.And then we analyzed the evolution,chromosome distribution and gene structure of the family genes and their gene expression patterns in different tissues during soybean development,and the expression of the family genes under low phosphorus stress.The results showed that the gene family was distributed in the whole soybean genome except chromosome 4,among which chromosome 3,10,13,18 and 19 were more distributed.The gene structure analysis showed that the whole family had a typical AP2 domain.Homologous sequence alignment and evolutionary analysis divided the genes of this family into 4 groups,including 15,5,19 and 20 genes,respectively.Tissue expression analysis based on Soybase database showed that the family genes were expressed in different tissues of soybean.qRT-PCR results showed that the family genes were induced in soybean roots,stems and leaves under low phosphorus stress.The results showed that the structure of soybean ERF genes were conservative,and were involved in the response to low phosphorus stress.