摘要
为了解决大豆种植过程中因过量施用化肥带来的土壤问题,从真菌多样性和组成结构角度评估微生物菌肥对大豆根际真菌群落的影响,本研究通过减施化肥配施微生物菌肥等不同处理,采用高通量测序技术研究大豆花期根际土壤真菌多样性和结构组成的变化。结果表明:子囊菌门(Ascomycota)、被孢霉菌门(Mortierellomycota)和担子菌门(Basidiomycota)为主要菌门,其中子囊菌门为主要优势菌类群。同一地块不同施肥方式,其根际真菌群落存在差异,减施化肥配施微生物复合菌肥可以有效降低镰刀菌属丰度,单施微生物菌肥可有效提高青霉属(Penicillium)和曲霉科(Aspergillaceae)丰度。综上,微生物菌肥可以增加有益菌丰度,减少病原菌丰度。
In order to solve the soil problems caused by excessive application of chemical fertilizer in the process of soybean planting,and evaluate the impact of microbial bacterial fertilizer on soybean rhizosphere fungal community from fungal diversity and composition structure,this study studied the changes of fungal diversity and structural composition in soybean rhizosphere soil at flowering stage by reducing the application of chemical fertilizer and microbial bacterial fertilizer.The results showed that Ascomycota,Mortierellomycota and Basidiomycota were the main flora,and Ascomycota was the main dominant flora.There were differences in rhizosphere fungal communities in the same plot with different fertilization methods.Reducing chemical fertilizer combined with microbial compound bacterial fertilizer can effectively reduce the abundance of Fusarium,and applying microbial bacterial fertilizer alone can effectively improve the abundance of Penicillium and aspergillaceae.In conclusion,microbial fertilizer can increase the abundance of beneficial bacteria and reduce the abundance of pathogenic bacteria.
作者
胡基华
张烨
张淑梅
陈静宇
姜威
曹旭
李晶
吴皓琼
HU Ji-hua;ZHANG Ye;ZHANG Shu-mei;CHEN Jing-yu;JIANG Wei;CAO Xu;LI Jing;WU Hao-qiong(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,China;Institute of Advanced Technology,Heilongjiang Academy of Sciences,Harbin 150020,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2021年第6期805-812,共8页
Soybean Science
基金
黑龙江省应用技术研究与开发计划(A19B105)。
关键词
微生物菌肥
土壤微生物
根际真菌多样性
减施化肥
ITSrDNA
Microbial fertilizer
Soil microbes
Rhizosphere fungal diversity
Reduced application of chemical fertilizers
ITSrDNA