基于荧光动力学技术的黄渤海初级生产力快速测量

Fast Measurement of Primary Productivity in the Yellow Sea and Bohai Sea Based on Fluorescence Kinetics Technology

浮游植物初级生产作为海洋生态系统的重要起点,是海洋食物网的基础,是衡量海水质量的重要指标。为了准确测量、监测和预测浮游植物初级生产力的时空变化及其对外部环境条件的动力学响应,研究了一种基于叶绿素荧光动力学的测量技术,研制出响应时间为1.6min、精度为4.86%的快速测量仪器。仪器测得的浮游植物初级生产力与液相氧电极法测得的光合放氧速率的相关系数为0.991。该仪器装备在中国海监101号实验船上,用于黄渤海浮游植物初级生产力在线测量和垂直剖面测量。测量结果表明,渤海湾内的浮游植物初级生产力是湾外的1.1~1.4倍,垂直剖面测量结果显示黄海区域各点位的浮游植物初级生产力的均与深度相关。与传统方法相比,所研制的仪器具有快速、稳定、无需样品培养等优点,可为海洋生态环境评价提供先进的技术方法。

As an important-starting point in the marine ecosystem,phytoplankton primary production is the foundation of the marine food web and an important indicator for seawater quality.In this paper,in order to accurately measure,monitor,and predict spatiotemporal variations of phytoplankton primary productivity,and its kinetic response to external environmental conditions,a measurement technology based on chlorophyll fluorescence kinetics was studied,and a fast measuring instrument with a response time of 1.6 minutes and a precision of 4.86%was developed.The correlation coefficient between the measured phytoplankton primary productivity by the developed instrument and the measured photosynthetic oxygen evolution rate by the liquidphase oxygen electrode was 0.991.The instrument was equipped on the China Marine Surveillance 101 experimental ship for the underway measurement and the vertical profiles measurement of phytoplankton primary productivity in the Yellow Sea and the Bohai Sea.Underway measurement results showed that the phytoplankton primary productivity in Bohai bay is 1.1~1.4 times higher than outside the bay,and vertical profiles measurement results showed a depth-dependent response of phytoplankton primary productivity on each site in the Yellow sea area.Compared with traditional methods,the developed instrument in this paper has the advantages of rapidness,stability,and no need for sample cultivation and can provide an advanced technical method for marine ecological environment assessment.