水通道蛋白1与非小细胞肺癌的相关性研究

Study on the Correlation Between AQP1 and Non-small Cell Lung Cancer

目的探讨水通道蛋白-1(AQP1)在体外对非小细胞肺癌细胞的影响及AQP1在非小细胞肺癌胸膜组织中的表达情况,分析其潜在应用价值。方法收集广西医科大学第一附属医院2020年5月-2021年10月内科胸腔镜下组织标本60例,其中非小细胞肺癌胸膜组织标本40例,良性胸腔胸膜组织标本20例进行石蜡包埋切片,采用免疫组化S-P法测定AQP1的表达;通过转染慢病毒及筛选,获得过表达AQP1的稳定株,利用细胞增殖实验检测转染过表达AQP1慢病毒对A549细胞增殖能力的影响,通过流式细胞仪检测抑制AQP1对A549细胞凋亡能力的影响。结果免疫组化显示,AQP1在非小细胞肺癌胸膜组织中呈高表达,高于良性胸膜组织,差异有统计学意义(P<0.05);细胞增殖实验和凋亡实验显示,与正常细胞相比过表达AQP1可以增强A549细胞的增殖能力,抑制AQP1的表达则促进了A549细胞早期和晚期的凋亡率。结论AQP1在非小细胞肺癌患者胸膜组织中高表达且AQP1可能通过抑制凋亡而促进肺癌细胞增殖。

Objective To explore the effect of Aquaporin1 on non-small cell lung cancer cells in vitro and the expression of AQP1 in pleural tissues of non-small cell lung cancer,and to analyze its potential clinical application value.Methods A total of 60 cases of thoracoscopic tissue specimens were collected from the First Affiliated Hospital of Guangxi Medical University from May 2020 to October 2021,including 40 cases of non-small cell lung cancer pleural tissue specimens and 20 cases of benign pleural tissue specimens.Paraffin-embedded sections were used to detect the expression of AQP1 by immunohistochemical S-P method.The stable AQP1 overexpression strain was obtained by transfecting lentivirus and screening.The effect of AQP1 overexpression lentivirus on the proliferation of A549 cells was detected by cell proliferation assay.The effect of AQP1 inhibition on the apoptosis of A549 cells was detected by flow cytometry.Results Immunohistochemistry showed that AQP1 was highly expressed in pleural tissue of non-small cell lung cancer,which was higher than that in benign pleural tissue(P<0.05).Cell proliferation assay and apoptosis assay showed that overexpression of AQP1 could enhance the proliferation of A549 cells compared with normal cells.Inhibition of AQP1 expression promoted the early and late apoptosis of A549 cells.Conclusion AQP1 is highly expressed in pleural tissue of patients with non-small cell lung cancer and AQP1 may promote the proliferation of lung cancer cells by inhibiting apoptosis.