双歧杆菌制剂对大鼠肿瘤模型化疗引起肠道炎症的影响

Effect of Bifidobacterium Preparation on Intestinal Inflammation Induced by Chemotherapy in Rat Tumor Model

目的探讨双歧杆菌对减少氟尿嘧啶化疗过程中血流感染的价值。方法选择健康SD大鼠24只,建立肿瘤模型后随机分为A、B、C、D4组,每组6只,A组作为对照,B组给予氟尿嘧啶,C组给予氟尿嘧啶+低剂量双歧杆菌,D组给予氟尿嘧啶+高剂量双歧杆菌,观察各组大鼠的体重、活动、饮食情况及粪便隐血情况,7 d后解剖观察肠道充血、水肿、溃疡情况,检测MPO、NF-κB活性。结果B、C、D组大鼠体重均小于A组,差异有统计学意义(P<0.05),但D组大鼠体重高于B组,差异有统计学意义(P<0.05);B组粪便细菌染色的球杆比高于A组,D组粪便细菌染色的球杆比高于C组,差异有统计学意义(P<0.05);A组粪便隐血、肠组织MPO和NF-κB低于B、C、D组,B组隐血、MPO和NF-κB低于C、D组,差异有统计学意义(P<0.05);HE染色显示,A组无明显炎性细胞浸润,基本正常结肠组织结构;B组肠组织的黏膜、黏膜下炎症细胞重度浸润;C组黏膜下层炎症明显细胞浸润,腺体减少;D组黏膜下轻度炎症细胞浸润。结论在肿瘤化疗过程中加入大剂量的双歧杆菌制剂可以减少肠道炎症和血流感染的风险,最终可提高治疗成功率。

Objective To investigate the value of Bifidobacterium in reducing blood flow infection during fluorouracil chemotherapy.Methods Twenty-four healthy SD rats were randomly divided into groups A,B,C and D after the establishment of the tumor model,with six rats in each group.Group A was used as the control,group B was given fluorouracil,group C was given fluorouracil+low-dose Bifidobacterium,group D was given fluorouracil+high-dose Bifidobacterium.The weight,activity,diet and fecal occult blood of rats in each group were observed.After 7 days,intestinal congestion,edema and ulcer were observed,and MPO and NF-κB activities were detected.Results The body weight of rats in group B,C and D was lower than that in group A,the difference was statistically significant(P<0.05),but the body weight of rats in group D was higher than that in group B,the difference was statistically significant(P<0.05).The ratio of fecal bacteria staining in group B was higher than that in group A,and the ratio of fecal bacteria staining in group D was higher than that in group C(P<0.05).The fecal occult blood,intestinal MPO and NF-κB in group A were lower than those in groups B,C and D,while the fecal occult blood,intestinal MPO and NF-κB in group B were lower than those in groups C and D,and the differences were statistically significant(P<0.05).HE staining showed that there was no obvious inflammatory cell infiltration and normal colon tissue structure in group A;severe infiltration of mucosal and submucosal inflammatory cells in intestinal tissue of group B;submucosa inflammation in group C was significantly cell infiltration,glands decreased;submucosal mild inflammatory cell infiltration in group D.Conclusion The addition of large doses of Bifidobacteria preparation during tumor chemotherapy can reduce the risk of intestinal inflammation and blood flow infection,and ultimately improve the success rate of treatment.