摘要
目的探讨慢性背根神经节受压(CCD)对大鼠脊髓背角Wnt/β-catenin信号通路的影响。方法选取42只成年雄性SD大鼠,按照随机数字表法将其分为假手术组和CCD组,其中假手术组9只,CCD组33只。按照术后时间点不同,将CCD组细分为术后1 d组(6只)、术后3 d组(6只)、术后7 d组(9只)、术后14 d组(6只)、术后28 d组(6只)。术前及术后1 d、3 d、5 d、7 d、14 d、21 d、28 d,检测各组大鼠机械缩足阈值。术后1 d、3 d、7 d、14 d、28 d,采用Western blot技术检测各组大鼠脊髓背角中活化的β-连环蛋白(activeβ-catenin)及胶质纤维酸性蛋白(GFAP)的表达情况;术后7 d,利用免疫荧光技术检测大鼠脊髓背角中activeβ-catenin的核转位及星形胶质细胞的活化情况。结果术后1 d、3 d、5 d、7 d、14 d、21 d、28 d,CCD组大鼠机械缩足阈值均显著低于假手术组(P<0.05);Western blot显示CCD术后各时间点脊髓背角中activeβ-catenin表达均较假手术组升高(P<0.05),CCD术后7 d,大鼠脊髓背角中GFAP的表达较假手术组明显升高(P<0.05)。免疫荧光结果显示,CCD术后脊髓背角中activeβ-catenin表达增多且发生核转位,星形胶质细胞发生明显的活化。结论Wnt/β-catenin信号通路在CCD模型大鼠脊髓背角中显著激活,提示其可能在神经病理性疼痛发生发展中发挥重要作用。
Objective To investigate the effect of chronic compression of the dorsal root ganglion(CCD)on the Wnt/β-catenin signaling pathways in the spinal dorsal horns of rats.Methods Forty-two adult male Sprague-Dawley rats were randomly divided into a sham group(n=9)and a CCD group(n=33).The CCD group was subdi-vided into a 1d group(n=6),a 3d group(n=6),a 7d group(n=9),a 14d group(n=6),and a 28d group(n=6)based on the post-operative time of the experiments.Before the operation for CCD and 1,3,5,7,14,21 and 28 days afterward the mechanical withdrawal threshold was detected for all rats.Western blotting was conducted to detect the expression of activeβ-catenin and glial fibrillary acidic protein(GFAP)in the dorsal horn of the spinal cord 1,3,7,14 and 28 days after the surgery.Seven days after the operation immunofluorescence was employed to detect the nuclear translocation of activeβ-catenin and the activation of astrocytes in the dorsal horn of the spinal cord.Results The average mechanical withdrawal thresholds of the CCD groups were significantly lower than that of the sham group at each time point.The western blotting showed that the expression of activeβ-catenin in the CCD groups was signifi-cantly greater than in the sham group at each time point.Seven days after compression the expression of GFAP in the rats′dorsal horns was significantly higher than in the sham group.Immunofluorescence indicated nuclear translocation of activeβ-catenin and the activation of astrocytes in the dorsal horn.Conclusion The Wnt/β-catenin signaling pathways are significantly activated in the dorsal horn of the spinal cord after CCD,at least in rats.It may play an im-portant role in the development of neuropathic pain.
作者
张也
陈雪梅
许东升
Zhang Ye;Chen Xuemei;Xu Dongsheng(Department of Rehabilitation,The Sixth People′s Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China;Department of Human Anatomy,Basic Medical College of Zhengzhou University,Zhengzhou 450001,China;School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2022年第2期97-102,共6页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金面上项目(81772453,81974358)。
