分子印迹技术在黄曲霉毒素检测中的最新研究进展

Recent research advances in aflatoxin detection by molecular imprinting technique

黄曲霉毒素(AFT)是由黄曲霉和寄生曲霉等某些菌株产生的一类真菌毒素,主要包括B1,B2,G1,G2,M1,M2,GM,P1,Q1,毒醇等,其中B1的毒性最大,致癌性最强,其广泛存在土壤、动植物、各种坚果、谷物,奶,食用油及其制品中,因此,食品中超痕量及痕量的黄曲霉毒素的检测方法受到高度关注。由于农产品样品基质复杂,而黄曲霉毒素含量和限量极低,高效特异性样品前处理技术是高灵敏精准检测黄曲霉毒素的关键之重。近年来分子印迹技术在样品前处理和核心识别元件等方面发展迅猛,在黄曲霉毒素痕量检测方面凸显出一定优势。综述了近几年来分子印迹技术在黄曲霉毒素前处理及快速检测等方面最新研究进展,并展望了分子印迹技术在黄曲霉检测应用的发展前景。

Aflatoxin(AFT) is a kind of fungus toxin produced by certain strains of aspergillus flavus and parasitic aspergillus,mainly including B1,B2,G1,G2,M1,M2,GM,P1,Q1,alcohol,etc.Among them,B1 is the most toxic and carcinogenic one and is wide spread in the soil,plants,animals,all kinds of nuts,grains,milk,cooking oil and its products.Therefore,detection of trace amount of AFT in food has been attracting attentions.Due to the complex substrates of agricultural product samples and the extremely low content of AFT,the highly efficient and specific sample pretreatment technology is the key to the highly sensitive and accurate detection of AFT.In recent years,molecular imprinting technology has developed rapidly in sample pretreatment and core identification elements,showing certain advantages in trace detection of aflatoxin.In this paper,the recent advances of molecular imprinting technology in the pretreatment and rapid detection of AFT were reviewed,and the development trend of molecular imprinting technology in the detection of aspergillus flavus was prospected.