LncRNA FGD5-AS1通过miR-873-5p/GTPBP4轴促进肝细胞癌发展的研究

The research of LncRNA FGD5-AS1 promotes hepatocellular carcinoma progression through miR-873-5p/GTPBP4 axis

目的探讨长链非编码RNA(lncRNA)FGD5-AS1对肝细胞癌(HCC)发展的调控机制。方法通过RT-qPCR检测FGD5-AS1在肝癌组织和肝癌细胞系中的表达水平。采用CCK-8、EdU、流式细胞术、细胞划痕实验和Transwell实验检测FGD5-AS1对HCC细胞增殖、凋亡、迁移和侵袭中的作用。运用双荧光素酶报告和RNA下拉实验探明FGD5-AS1、miR-873-5p和GTPBP4之间的相互作用。结果FGD5-AS1在肝癌组织和细胞中表达上调。FGD5-AS1表达下调可抑制HCC细胞的增殖、迁移和侵袭,并诱导其凋亡。FGD5-AS1直接与miR-873-5p结合,并竞争性抑制其表达,以提高HCC细胞中促癌基因GTPBP4的表达水平。FGD5-AS1的作用是由miR-873-5p/GTPBP4轴介导的。结论FGD5-AS1可通过调控miR-873-5p/GTPBP4轴从而促进HCC细胞的增殖、迁移和侵袭,并抑制其凋亡。

Objective To explore the regulatory mechanism of the long noncoding FGD5-AS1(LncRNA FGD5-AS1)on hepatocellular carcinoma(HCC)progression.Methods The expression level of FGD5-AS1 was measured in tumor tissues and cell lines by RT-qPCR.CCK-8,EdU,flow cytometry,wound healing and transwell chamber assays were performed to investigate the role of FGD5-AS1 in HCC cell proliferation,apoptosis,migration and invasion in vitro.At the molecular level,dual luciferase reporter and RNA pull down assays were performed to identify the interaction among FGD5-AS1,miR-873-5p and GTPBP4.Results FGD5-AS1 was upregulated in HCC tissues and cells.Moreover,FGD5-AS1 knockdown suppressed HCC cell proliferation,migration and invasion,and induced apoptosis in vitro.Mechanistically,FGD5-AS1 directly bound to miR-873-5p and competitively inhibit its expression to increase the expression level of GTPBP4 in HCC cells.Finally,our findings indicated that the role of FGD5-AS1 was mediated by miR-873-5p GTPBP4 axis.Conclusion FGD5-AS1 promotes the proliferation,migration and invasion of HCC cells and inhibits apoptosis by regulating the miR-873-5p/GTPBP4 axis.