滑膜肉瘤中P300与EMT相关蛋白的表达及临床意义

The clinical significance of P300 and EMT-related proteins in synovial sarcoma

目的通过检测滑膜肉瘤(SS)组织中组蛋白乙酰转移酶P300及上皮间叶转化(EMT)相关分子的表达,探讨P300在SS双相分化及EMT中的作用及临床意义。方法收集40例SS石蜡包埋组织样本,运用RT-PCR方法检测SS的SYT-SSX融合基因亚型;免疫组化检测P300及EMT相关分子的表达。结果①40例SS中[双相型滑膜肉瘤(BSS)30例,单相型滑膜肉瘤(MFSS)10例],37例存在SYT-SSX融合基因(包括SYT-SSX1、SYT-SSX2),其中BSS为90%(27/30)(SYT-SSX166.7%,SYT-SSX233.3%);MFSS为100%(10/10)(SYT-SSX180%,SYT-SSX220%),SS组织学类型与融合基因类型的差异无统计学意义。②95%(38/40)的SS病例表达P300,包括93.3%(28/30)的BSS以及100%(10/10)的MFSS,P300的表达与SS组织学类型的差异无统计学意义。③P300与SS患者临床病理参数间的差异无统计学意义;但COX回归分析显示肿瘤转移(P=0.019)和TNM分期(Ⅲ~Ⅳ)(P=0.003)是患者总体生存时间的独立危险因素。④BSS中P300阳性的病例与EMT相关蛋白β-catenin的表达差异有统计学意义(P=0.027);MFSS中P300阳性的病例与EMT相关蛋白Slug的表达差异有统计学意义(P=0.048),提示P300与EMT密切相关。结论P300可能参与SS的双相分化。P300可能参与SS的EMT过程,提示P300在SS的侵袭迁移中发挥了一定作用。

Objective To explore the role and its clinical significance of P300 in biphasic differentiation and epithelial mesenchymal transition(EMT)process in synovial sarcoma(SS)by detecting the expression of histone acetyltransferase P300 and EMT related molecules.Methods 40 cases of SS paraffin embedded tissue samples were collected,and the SYT-SSX fusion gene subtype was detected by RT-PCR.Immunohistochemistry was used to detect the expression of P300 and EMT-related molecules.Results①The positivity of SYT-SSX fusion gene was 92.5%(37/40),including 90%(27/30)of BSS(66.7%SYT-SSX1,33.3%SYT-SSX2)and 100%(10/10)of MFSS(SYT-SSX180%,SYT-SSX220%),there was no correlation between histological subtype and fusion gene subtype in SS.②The rate of positive expression of P300 was 95%(38/40),including 93.3%(28/30)of BSS and 100%(10/10)of MFSS,and there was no significance difference between the expression P300 and histological subtype.③The expression of P300 was not statistically correlated with clinicopathological parameters of SS,however,COX regression analysis showed that tumour metastasis(P=0.019)and TNM stage(Ⅲ~Ⅳ)(P=0.003)were independent risk factors for overall survival of patient.④The expression of P300 was significantly related with EMT-associated proteinβ-catenin in the BSS(P=0.027)and Slug in the MFSS(P=0.048),which suggested P300 was closely related to EMT.Conclusion P300 may be involved in the biphasic differentiation and the EMT process of SS,suggesting that P300 plays a certain role in SS invasion and migration.