SPIB对食管癌增殖及上皮间质转化的影响

Effects of SPIB on proliferation and epithelial-stromal transformation of esophageal carcinoma

目的通过调控食管癌细胞内转录因子SPIB蛋白表达,研究SPIB对食管癌细胞增殖、迁移及侵袭的作用及机制。方法采用Western blot和qRT-PCR方法检测食管癌细胞系中SPIB mRNA和蛋白表达。通过siRNA下调食管癌细胞系KYSE150细胞内SPIB表达,采用CCK-8和流式细胞术检测细胞增殖和细胞周期。建立稳定过表达SPIB的KYSE30细胞系,检测SPIB对细胞增殖和克隆形成能力的影响。采用细胞划痕和Transwell法系统检测SPIB对食管癌细胞迁移和侵袭能力的影响,采用Western blot法检测细胞内N-cadherin和E-cadherin蛋白的表达水平。结果SPIB在食管癌细胞系中呈高表达,下调其表达可抑制细胞增殖能力和细胞周期进程,并减弱细胞内Cyclin D1蛋白表达。过表达SPIB则促进食管癌细胞增殖和克隆形成。敲低SPIB蛋白表达可减弱食管癌细胞迁移和侵袭能力,并减弱了N-cadherin蛋白的表达。结论SPIB作为一种促癌因子,可促进食管癌细胞增殖、迁移和侵袭。

Objective To study the effect and mechanism of transcription factor SPIBon proliferation,migration and invasion of esophageal cancer cells after regulating the expression of SPIB protein by gene expression regulation technology.Methods The expression levels of SPIB mRNA and protein in esophageal cancer cell lines were detected by Western blot and qRT-PCR,respectively.SPIB expression was down-regulated by siRNA in KYSE150 cells,and the proliferation and cell cycle progression were detected by Cell Counting Kit-8 and flow cytometry,respectively.The KYSE30 cell lines with stable overexpression of SPIB were established and the effects of SPIB on cell proliferation and clone formation were detected.The effects of SPIB on the migration and invasion of esophageal cancer cells were detected by cell scratch and Transwell system,and the expression levels of N-cadherin and E-cadherin proteins were detected by Western blot.Results SPIB was highly expressed in esophageal cancer cell lines.Down-regulation of SPIB could inhibit cell proliferation and cell cycle progression,and decrease the expression of Cyclin D1 proteins.Overexpression of SPIB enhanced the proliferation and clone formation of esophageal cancer cells.Knockdown of SPIB protein expression weakened the migration and invasion ability of esophageal cancer cells,and reduced the expression of N-cadherin protein.Conclusion SPIB,as a pro-cancer factor,can promote the proliferation,migration and invasion of esophageal cancer cells.