新疆哈萨克族食管鳞癌差异表达mRNA的筛选

Identifying the differentially expressed mRNAs in esophagus squamous cell carcinoma of Kazakh patients in Xinjiang

目的对新疆哈萨克族食管鳞癌(ESCC)组织与正常组织间差异表达的mRNA进行基因芯片筛选,进一步了解ESCC发生、发展机制。方法利用基因芯片对5对新疆哈萨克族ESCC组织与癌旁正常组织(距癌组织>5 cm)中mRNA进行筛选;利用R软件及R软件包进行生物信息学分析;结合生物信息学工具metascape对差异基因行GO功能注释和KEGG通路富集分析;利用生物信息学网站STRING及Cytoscape软件寻找核心基因;搜集23例新疆哈萨克族ESCC组织和20例癌旁正常组织,对核心基因PLAUR进行免疫组化验证。结果最终筛选出哈萨克族ESCC差异表达的mRNA 1764个(差异倍数≥2且P<0.01);差异表达的mRNA中上调的基因378个,下调的基因1368个,这些差异表达的mRNA涉及了一系列生物学功能及通路;免疫组化结果显示核心基因PLAUR在哈萨克族ESCC中表达高于癌旁正常组织。结论该研究通过基因芯片筛选出新疆哈萨克族ESCC组织与癌旁正常组织中差异表达的mRNA,找出了其中的核心基因,完善了新疆哈萨克族ESCC基因谱,为ESCC诊断、预后的进一步研究打下基础。

Objective To screen the differentially expressed mRNA between esophageal squamous cell carcinoma(ESCC)and normal tissues of Kazakh in Xinjiang by microarray,so as to further understand the occurrence and development mechanism of ESCC.Methods Five pairs of esophageal squamous cell carcinoma and adjacent normal tissues(more than 5 cm away from cancer tissues)were screened by gene chip;Bioinformatics analysis was performed by R software and R software package;Metascape was used to carry out gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses;bioinformatics website String and Cytoscape software was used to find core genes;23 cases of esophageal squamous cell carcinoma(ESCC)and 20 cases of adjacent normal tissues of Kazakh in Xinjiang were collected,and the core gene PLAUR was verified by immunohistochemistry.Results 1764 differentially expressed mRNAs were screened out(multiple of difference≥2 and P<0.01);Among the differentially expressed mRNAs,378 genes were up-regulated and 1368 genes were down regulated.These differentially expressed mRNAs involved a series of biological functions and pathways;Immunohistochemical results showed that the expression of the core gene PLAUR in Kazakh esophageal squamous cell carcinoma was higher than that in adjacent normal tissues.Conclusion The differentially expressed mRNAs in esophageal squamous cell carcinoma and adjacent normal tissues of Kazakh in Xinjiang was screened by microarray,and the core genes were found out,which improved the gene spectrum of esophageal squamous cell carcinoma of Kazakh in Xinjiang,and laid a foundation for further research on the diagnosis and prognosis of esophageal squamous cell carcinoma.