摘要
采用健康人外周血体外诱导B淋巴细胞增殖分化为浆母细胞及浆细胞。通过观察细胞形态特征,流式细胞术分析B细胞膜表面分子表达水平及细胞活力,发现CD40L信号激发、CpG免疫调节序列联合及优化细胞因子组合运用,能有效促进人B淋巴细胞的转化及体外建系。
Peripheral blood of healthy people was used to induce the proliferation and differentiation of B lymphocytes into plasmablasts and plasma cells in vitro.Cells characteristics were observed under microscope.Molecules expression level of B cells surface was analyzed under flow cytometry.Cell viability was observed by Trypan blue.The result showed CD40L signal stimulation,CpG immunoregulatory sequence combination and optimized cytokine combination could effectively promote the transformation of human B lymphocytes and the establishment of vitro model.
作者
刘静
陈惠荣
郭佳琦
马金海
Liu Jing;Chen Huirong;Guo Jiaqi;Ma Jinhai(Dept of Pediatrics,General Hospital of Ningxia Medical University,Yinchuan 750004;Laboratory of Ningxia Medical University,Yinchuan 750004)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第3期493-496,共4页
Acta Universitatis Medicinalis Anhui
基金
国家留学基金(留金项[2018]10038号)
宁夏自治区自然科学基金(编号:2019AAC03186)。
关键词
B细胞
浆母细胞
浆细胞
体外
B cells
plasmablasts
plasma cells
in vitro
