醋酸阿比特龙对戈舍瑞林去势抵抗性前列腺癌细胞化疗敏感性的影响

Effect of abiraterone acetate on chemosensitivity of goserelin castration-resistant prostate cancer cells

目的探讨醋酸阿比特龙(AA)对戈舍瑞林去势抵抗性前列腺癌(CRPC)PC3细胞化疗敏感性的影响。方法 CRPC PC3细胞分为空白组、对照组、实验组和联合组,用不含药、含20%致死浓度(IC_(20))多西他赛、含IC;AA、多西他赛IC_(20)AA+IC_(20)多西他赛的细胞培养液进行干预。用噻唑蓝法检测细胞的增殖情况,用流式细胞术检测细胞的凋亡能力。结果空白组、对照组、实验组和联合组培养48 h的增殖能力[光密度(OD)值]分别为0.52±0.06,0.41±0.05,0.41±0.05和0.25±0.04,培养72 h的增殖能力(OD值)分别为0.82±0.09,0.63±0.06,0.63±0.07和0.44±0.05,培养48 h的细胞凋亡率分别为(5.81±1.30)%,(14.71±2.66)%,(15.35±2.60)%和(24.61±3.50)%。联合组的上述指标与对照组和实验组比较,差异均有统计学意义(均P<0.05)。结论 AA可抑制戈舍瑞林CRPC PC3细胞的增殖,促进凋亡,增强其对多西他赛的敏感性。

Objective To investigate the effect of abiraterone acetate(AA) on the chemotherapy sensitivity of goserelin castration-resistant prostate cancer(CRPC) PC3 cells. Methods CRPC PC3 cells were divided into blank, control, experimental and combined groups, and they were treated with drug-free, 20% lethal concentration(IC_(20)) AA, IC_(20) docetaxel, IC_(20)AA+IC_(20) docetaxel cell culture medium, respectively. Methyl thiazolyl tetrazolium and flow cytometry were used to detect the cell proliferation and apoptosis. Results The optical density(OD) values of proliferation capacity for 48 h in the blank, control, experimental and combined groups were 0.52±0.06, 0.41±0.05, 0.41±0.05 and 0.25±0.04, the OD values of proliferation capacity for 72 h were 0.82±0.09, 0.63±0.06, 0.63±0.07 and 0.44±0.05, apoptosis rates for 48 h were(5.81±1.30)%,(14.71±2.66)%,(15.35±2.60)% and(24.61±3.50)%. Compared with combined group, the above indexes of control and experimental groups were statistically significant(all P<0.05). Conclusion AA can effectively inhibit the proliferation of goserelin CRPC PC3 cells, promote apoptosis and enhance the sensitivity of docetaxel.