极光激酶B、肿瘤蛋白53在肝细胞癌中的表达及与临床病理特征、预后的关系

The expression and clinical significance of AURKB and TP53 in hepatocellular carcinoma

目的 探究极光激酶B(AURKB)、肿瘤蛋白53(TP53)在肝细胞癌(HCC)中的表达情况及其与临床病理特征、预后的关系。方法 Ualcan数据库分析AURKB、TP53在正常肝组织和HCC组织中的表达情况。选取2015年4月至2018年1月空军军医大学第一附属院医院诊治的95例HCC患者(肿瘤Ⅱ期)为研究对象。实时荧光定量PCR检测HCC组织、癌旁正常组织AURKB、TP53 mRNA表达水平。分析HCC组织AURKB、TP53 mRNA表达水平与临床病理特征关系;Pearson法分析HCC组织中AURKB mRNA表达水平与TP53 mRNA的相关性;Kaplan-Meier生存曲线分析HCC组织AURKB、TP53 mRNA表达水平与HCC患者预后的关系;Cox回归分析HCC患者预后的影响因素。结果 Ualcan数据库中HCC组织AURKB、TP53 mRNA表达水平为5.00(2.12,10.575)和19.91(12.77,29.72),高于正常肝组织的0.27(0.19,0.88)和13.37(11.24,15.29),(P<0.05)。HCC组织AURKB、TP53 mRNA表达水平为(1.82±0.61)和(1.75±0.59),高于癌旁正常组织的(1.01±0.34)和(1.04±0.35),(P<0.05)。HCC组织AURKB、TP53 mRNA表达水平与病灶转移呈正相关(P<0.05),与肿瘤分化程度呈负相关(P<0.05);HCC组织中AURKB mRNA表达水平与TP53 mRNA呈正相关(r=0.577,P<0.05);AURKB、TP53高表达组HCC患者术后36个月累积生存率低于AURKB、TP53低表达组(P<0.05);病灶转移、AURKB、TP53均是影响HCC患者不良预后的独立危险因素(P<0.05)。结论 HCC患者癌组织AURKB、TP53 mRNA表达水平较高,与临床病理特征、预后相关,AURKB、TP53有望成为评估HCC患者预后的指标。

Objective To explore the expression of Aurora kinase B(AURKB)and tumor protein P53(TP53)in hepatocellular carcinoma(HCC)tissues and their relationship with the clinicopathological characteristics and prognosis of the HCC patients.Methods Ualcan database was used for analyzing the expression of AURKB and TP53 in normal liver tissues and HCC tissues.A total of 95 HCC patients with stage II tumors who were diagnosed and treated from April 2015to January 2018 were selected as the research objects.Real-time fluorescent quantitative PCR(qRT-PCR)method was used to detect the mRNA expression levels of AURKB and TP53 in HCC and the adjacent normal tissues.Chi-square test was used to analyze the relationship between the mRNA expression levels of AURKB and TP53 in HCC tissues and clinicopathological characteristics;Pearson method was used to analyze the correlation between AURKB and TP53 mRNA expression levels in HCC tissues;Kaplan-Meier survival curve was used to analyze the relationship between the mRNA expression levels of AURKB and TP53 in HCC tissues and the prognosis of HCC patients;COX regression analysis was used to identify the factors affecting the prognosis of HCC patients.Results The mRNA expression levels of AURKB and TP53 in HCC tissues[5.00(2.12,10.575)、19.91(12.77,29.72)]in Ualcan database were higher than those in normal liver tissues[0.27(0.19,0.88)、13.37(11.24,15.29)](P<0.05).By Quantitative real time polymerase chain reaction(qRT-PCR)method it was found that the mRNA expression levels of AURKB and TP53 in HCC tissues[(1.82±0.61),(1.75±0.59)]were higher than those of the adjacent normal tissues[(1.01±0.34),(1.04±0.35)](P<0.05);the mRNA expression levels of AURKB and TP53in HCC tissues were positively correlated with tumor metastasis(P<0.05),and negatively correlated with the degree of tumor differentiation(P<0.05);the mRNA expression level of AURKB in HCC tissues was positively correlated with that of TP53(P<0.05);the 36-month cumulative survival rate of HCC patients in the AURKB and TP53 high expression groups was lower than that in the AURKB and TP53low expression groups(P<0.05);Lesion metastasis,AURKB and TP53 expression levels were all independent risk factors associated with the poor prognosis of HCC patients(P<0.05).Conclusion The mRNA expression levels of AURKB and TP53in HCC tissues are relatively higher than those of the adjacent normal tissues,both of which are related to the clinicopathological characteristics and prognosis of HCC patients.AURKB and TP53 are expected to be indicators for evaluating the prognosis of HCC patients.