枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理

Inhibitory Mechanism of Volatilized Active Components from Bacillus subtilis Czk1 on Ganoderma pseudoferreum

红根病是我国分布广、发病率高、危害较为严重的橡胶树根部病害。为明确枯草芽孢杆菌Czk1菌株产生的挥发性物质对橡胶红根病原菌橡胶灵芝菌(Ganoderma pseudoferreum)的抑菌作用,以挥发性物质的主要成分苯甲醛、3,5-二甲氧基苯乙酮、2,6-二叔丁基对甲酚为研究对象,分析其对橡胶灵芝菌的糖、蛋白、DNA、关键代谢酶活性、细胞内容物泄露情况的影响。结果表明,经挥发物混合活性组分处理后,橡胶灵芝菌糖类含量显著降低;高浓度的枯草芽孢杆菌Czk1菌株挥发物混合活性组分影响其蛋白质的合成;DNA分子量变化不明显,未破坏橡胶灵芝菌DNA的一级结构。关键代谢酶活性(苹果酸脱氢酶、几丁质酶)降低,细胞内溶物泄漏情况明显。挥发物混合活性组分可能是通过破坏橡胶灵芝菌的细胞膜结构,扰乱物质代谢活动,从而达到抑菌作用。

Red root disease is the root disease of rubber trees with wide distribution,high incidence,and more harmful in China.In order to determine the inhibitory effect of volatilized active components produced by Bacillus subtilis Czk1 strain on Ganoderma pseudoferreum,the dominant of active components,benzaldehyde,3,5-dimethoxyacetophenone and butylated hydroxytoluene were used as research subjects to analyze their effects on sugar,protein,DNA,activity of key metabolic enzymes and cell content leakage of G.pseudoferreum.The results showed after G.pseudoferreum was treated with the active components of volatiled organic compounds,the sugar content of G.pseudoferreum decreased significantly,and the volatilized active components with high concentration affected the protein synthesis of G.pseudoferreum;the molecular weight of DNA did not change significantly,indicating the primary structure of DNA was not destoried;the activities of key metabolic enzymes(malate dehydrogenase and chitinase)decreased,and the leakage of intracellular lysates was obvious.The active components of volatile organic compounds achieved bacteriostasis by destroying the cell membrane structure and disrupting the metabolic activity of G.pseudoferreum.