BTNL2、DVWA基因多态性与青海地区回族原发性膝骨关节炎的相关性

The Relationship between BTNL2 and DVWA Gene Polymorphisms and Primary Knee Osteoarthritis of the Hui Nationality in Qinghai

目的:探讨BTNL2、DVWA基因多态性与青海地区回族原发性膝骨关节炎的相关性。方法:选取2018年12月至2020年5月本院确诊膝骨关节炎患者100例作为病例组,同时选取同期健康体检人群100例作为对照组。通过标准聚合酶链反应和限制性片段长度多态性(PCR-RLFP)对BTNL2 rs10947262、DVWA rs11718863基因多态性进行检查,同时检查血清BTNL2、DVWA、hs-CRP、IL-4、IL-6、MMP-2、TNF-α、TG、HDL-C、LDL-C水平。分析BTNL2、DVWA基因多态性与原发性膝骨关节炎的相关性。结果:病例组血清BTNL2、DVWA、hs-CRP、IL-4、IL-6、MMP-2、TNF-α水平明显高于对照组(P<0.05)。病例组BTNL2 rs10947262 AA基因型比例高于对照组(62.00%vs 24.00%)(P<0.05);BTNL2 rs10947262 AA基因型能增加原发性膝骨关节炎易感性(OR=11.32、95%CI:5.96~22.98)(P<0.05)。病例组BTNL2 rs10947262 A等位基因频率比例高于对照组(76.00%vs 39.00%)(P<0.05);BTNL2 rs10947262 A等位基因频率能增加原发性膝骨关节炎易感性(OR=2.95、95%CI:2.45~3.72)(P<0.05)。病例组DVWA rs11718863 AA基因型比例高于对照组组(58.00%vs 20.00%)(P<0.05);DVWA rs11718863 AA基因型能增加原发性膝骨关节炎易感性(OR=10.26、95%CI:5.12~25.63)(P<0.05)。病例组DVWA rs11718863 A等位基因频率比例高于对照组(68.00%vs 50.00%)(P<0.05);DVWA rs11718863 A等位基因频率能增加原发性膝骨关节炎易感性(OR=2.69、95%CI:2.10~4.26)(P<0.05)。多因素Logistic回归分析发现:血清BTNL2、DVWA水平升高、BTNL2 AA基因型比例、BTNL2 A等位基因型比例、DVWA AA基因型比例、DVWA A等位基因型比例升高为原发性膝骨关节炎发生的危险因素(P<0.05)。结论:BTNL2 rs10947262 AA基因型、A等位基因频率,DVWA rs11718863 AA基因型、A等位基因频率增加可能与青海地区回族原发性膝骨关节炎易感相关。

Objective: To investigate the relationship between BTNL2 and DVWA gene polymorphisms and primary knee osteoarthritis of the Hui nationality in Qinghai. Methods: A total of 100 patients with knee osteoarthritis diagnosed in our hospital from December 2018 to May 2020 were selected as the case group, and 100 cases of healthy physical examination population during the same period were selected as the control group. BTNL2 rs10947262, DVWA rs11718863 gene polymorphisms were checked by standard polymerase chain reaction and restriction fragment length polymorphism(PCR-RLFP), and serum BTNL2, DVWA, hs-CRP, IL-4, IL-6,MMP-2, TNF-α, TG, HDL-C, LDL-C levels were checked at the same time. The correlations between BTNL2, DVWA gene polymorphisms and primary knee osteoarthritis were analyzed. Results: The serum levels of BTNL2, DVWA, hs-CRP, IL-4, IL-6, MMP-2 and TNF-α in the case group were significantly higher than those in the control group(P<0.05). The proportion of BTNL2 rs10947262 AA genotype in the case group was higher than that in the control group(62.00% vs 24.00%)(P<0.05), and BTNL2 rs10947262 AA genotype can increase the susceptibility of primary knee osteoarthritis(OR=11.32, 95% CI: 5.96~22.98)(P<0.05). The proportion of BTNL2 rs10947262 A allele frequency in the case group was higher than that in the control group(76.00% vs 39.00%)(P<0.05), and the BTNL2 rs10947262 A allele frequency could increase the susceptibility of primary knee osteoarthritis(OR=2.95, 95%CI: 2.45~3.72)(P<0.05).The proportion of DVWA rs11718863 AA genotype in the case group was higher than that in the control group(58.00% vs 20.00%)(P<0.05), and DVWA rs11718863 AA genotype can increase the susceptibility to primary knee osteoarthritis(OR=10.26, 95%CI: 5.12~25.63)(P<0.05). The DVWA rs11718863 A allele frequency ratio in the case group was higher than that in the control group(68.00% vs50.00%)(P<0.05), and the DVWA rs11718863 A allele frequency can increase the susceptibility of primary knee osteoarthritis(OR=2.69,95%CI: 2.10~4.26)(P<0.05). Multivariate logistic regression analysis found that: high levels of serum BTNL2, DVWA levels, BTNL2 AA genotype ratio, BTNL2 A allele genotype ratio, DVWA AA genotype ratio, DVWA A allele genotype ratio were risk factors for primary knee osteoarthritis(P<0.05). Conclusion: The increased frequency of AA genotype or A allele of BTNL2 rs10947262, AA genotype or A allele of DVWA rs11718863 may be related to the susceptibility of primary knee osteoarthritis in Qinghai area.