基于生物数据挖掘分析PTX3在非小细胞肺癌中的表达及其与预后的关系

Analysis of PTX3 Expression in Non-small Cell Lung Cancer and Its Relationship with Prognosis Based on Biological Data Mining

目的:探讨正五聚素蛋白3(PTX3)在非小细胞肺癌(NSCLC)中的表达及预后意义。方法:运用Oncomine、GEPIA分析PTX3在NSCLC组织中的表达情况,通过GEPIA分析PTX3表达与NSCLC患者生存期的相关性,利用CCLE分析PTX3在NSCLC细胞系中的表达水平,从CCLE下载NSCLC相关基因芯片并用R语言筛选PTX3共表达基因,利用基因本体(GO)和KEGG信号通路分析对PTX3相关共表达基因进行功能注释。结果:Oncomine和GEPIA数据库中分析显示PTX3基因在NSCLC组织中显著低表达(P<0.05);利用GEPIA数据库生存分析功能发现,PTX3高表达与NSCLC预后呈负相关(P<0.05);在CCLE数据库里利用R软件共筛选出105个NSCLC中与PTX3共表达的基因,GO功能富集分析表明,PTX3相关性蛋白主要定位于黏着斑、细胞-基质黏着连接及细胞间连接等,主要参与细胞外基质、细胞外结缔组织、细胞-基质粘附及上皮细胞发育等生物过程。KEGG分析显示PTX3共表达基因主要参与紧密连接、调节肌动蛋白骨架及JAK-STAT信号通路等。结论:PTX3基因在NSCLC组织中低表达,PTX3表达与NSCLC患者预后相关,可能作为NSCLC患者预后评估的分子标志物之一。

Objective To investigate the expression and prognostic significance of Pentraxin 3(PTX3)in non-small cell lung cancer(NSCLC).Methods:Oncomine and GEPIA were used to analyze the expression of PTX3 in NSCLC tissues.GEPIA was used to analyze the correlation between PTX3 expression and survival time of NSCLC patients.CCLE was used to analyze the expression level of PTX3 in NSCLC cell lines.NSCLC related gene chips were downloaded from CCLE and PTX3 co expressed genes were screened by R language.Gene Ontology(GO)and KEGG signaling pathway analysis were used to annotate PTX3 related coexpression genes.Results:Analysis of Oncomine and GEPIA databases showed significantly lower expression of PTX3 gene in NSCLC tissues(P<0.05).By using the GEPIA database survival analysis function,PTX3 high expression was negatively correlated with NSCLC prognosis(P<0.05).In CCLE database,using R software were screened in 105 NSCLC with PTX3 expression of genes,GO function enrichment analysis showed that correlation PTX3 protein mainly locate in gelling spot,cell-matrix adhesive connection and the connection between cells and so on,mainly involved in the extracellular matrix,extracellular connective tissue,cell-matrix adhesion and the development of epithelial cells such as biological processes.KEGG analysis showed that PTX3 co-expressed genes were mainly involved in tight binding,regulation of actin skeleton and JAK-STAT signaling pathway.Conclusions:PTX3 gene is lowly expressed in NSCLC tissue,and the PTX3 expression is associated with the prognosis of NSCLC patients,which may be used as one of the molecular markers for the prognosis evaluation of NSCLC patients.