HCMV-DNA定量检测和HCMV-IgG抗体AI检测在儿童HCMV感染诊断中的临床价值

Clinical Value of HCMV-DNA Quantitative Detection and HCMV-IgG Antibody AI Detection in the Diagnosis of HCMV Infection in Children

目的:探讨人巨细胞病毒(HCMV)-DNA定量检测和HCMV-免疫球蛋白G(Ig G)抗体亲和力指数(AI)检测在儿童HCMV感染诊断中的临床价值。方法:收集高度疑似HCMV活动性感染患儿血清样本103例作为研究组,健康体检儿童血清样本94例作为对照组。分析HCMV-DNA定量检测结果和HCMV-Ig G抗体AI检测结果,并比较不同年龄、不同性别患儿HCMV-DNA阳性结果检出率和低HCMV-Ig G抗体AI检出情况。结果:研究组血清HCMV-DNA阳性率为33.01%(34/103),对照组血清HCMV-DNA均为阴性,研究组血清HCMV-DNA阳性率明显高于对照组,差异有统计学意义(P<0.05)。研究组血清低HCMV-Ig G抗体AI检出率为13.59%(14/103),对照组未检出低HCMV-Ig G抗体AI,研究组血清低HCMV-Ig G抗体AI检出率高于对照组,差异有统计学意义(P<0.05)。研究组不同性别之间患儿血清的HCMV-DNA阳性率、低HCMV-Ig G抗体AI检测结果均无统计学差异(P>0.05)。研究组年龄1~5岁患儿血清HCMV-DNA阳性率明显低于年龄1 d~<6个月和年龄6个月~<1岁患儿(P<0.05)。三个年龄段患儿的血清低HCMV-Ig G抗体AI检测结果均无统计学差异(P>0.05)。结论:1岁以下儿童更易受到HCMV感染,HCMV-DNA定量检测和HCMV-Ig G抗体AI检测结果可以为临床早期诊断和治疗HCMV感染提供有效依据。

Objective:To investigate the clinical value of Human cytomegalovirus(HCMV)-DNA quantitative detection and HCMV-immunoglobulin G(Ig G)antibody avidity index(AI)detection in the diagnosis of HCMV infection in children.Methods:103cases of serum samples of children with highly suspected HCMV active infection were collected as the study group,and 94 cases of serum samples of healthy examination children were collected as control group.The HCMV-DNA quantitative detection results and HCMV-Ig G antibody AI detection results were analyzed.The detection rate of HCMV-DNA positive results and the detection of low HCMV-Ig G antibody AI in children of different ages and genders were compared.Results:The positive rate of serum HCMV-DNA in the study group was 33.01%(34/103),the serum HCMV-DNA in the control group was negative,while the positive rate of serum HCMV-DNA in the study group was significantly higher than that in the control group,with statistical significance(P<0.05).The detection rate of serum low HCMV-Ig G antibody AI in the study group was 13.59%(14/103),while low HCMV-Ig G antibody AI in the control group was not detected.The detection rate of serum low HCMV-Ig G AI in the study group was higher than that in the control group,with statistical significance(P<0.05).There were no significant differences in serum HCMV-DNA positive rate and low HCMV-Ig G antibody AI detection results in the different genders in the study group(P>0.05).In the study group,the positive rate of serum HCMV-DNA of children aged 1~5 years was significantly lower than that of children aged 1d~<6 months and 6 months~<1years(P<0.05).There was no significant difference in serum low HCMV-Ig G antibody AI detection among the three age groups(P>0.05).Conclusion:Children under 1 year old are more susceptible to HCMV infection.The results of HCMV-DNA quantitative detection and HCMV-Ig G antibody AI detection can provide effective basis for the early diagnosis and treatment of HCMV infection in clinical practice.