荜铃胃痛颗粒抗实验性胃溃疡作用研究

Effect of Biling Weitong Granules in treating experimental gastric ulcer

目的探究荜铃胃痛颗粒对乙醇诱导胃溃疡模型大鼠的保护作用。方法SD大鼠随机分为对照组、模型组及荜铃胃痛颗粒低、中、高剂量(0.79、1.58、3.16 g·kg^(−1))组和西咪替丁(42.00 mg·kg^(−1),阳性药)组,每组8只;各组均按剂量预给药8 d,对照组和模型组大鼠ig等体积0.5%羧甲基纤维素钠(CMC-Na)溶液。末次给药30 min后,除对照组外,其余大鼠ig给予1 mL无水乙醇造模,1 h后牺牲动物取材;展开胃黏膜面拍照,测量溃疡面积,取部分胃组织进行HE染色;检测血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和超氧化物歧化酶(SOD)水平,检测胃组织中髓过氧化物酶(MPO)和前列腺素E_(2)(PGE_(2))水平。结果与对照组比较,模型组大鼠胃溃疡面积和胃黏膜病理评分显著升高(P<0.001),大鼠血清中IL-1β(P<0.01)和TNF-α(P<0.05)水平均显著升高,大鼠胃组织MPO活力显著升高(P<0.001),大鼠胃组织中PGE_(2)水平显著降低(P<0.01)。与模型组比较,各给药组大鼠胃溃疡面积和胃黏膜病理评分显著降低(P<0.05、0.01、0.001)。与模型组比较,荜铃胃痛颗粒组大鼠血清IL-1β和TNF-α水平显著降低(P<0.05、0.001),胃组织中MPO活力显著降低(P<0.01、0.001),血清SOD活力显著升高(P<0.05);胃组织PGE_(2)水平显著增加(P<0.05)。结论荜铃胃痛颗粒可通过抑制炎症因子分泌、缓解机体氧化应激、保护胃黏膜等发挥对胃溃疡模型大鼠的保护作用。

Objective To explore the protective effect of Biling Weitong Granules on ethanol-induced gastric ulcer rats.Methods Sprague Dawley rats were randomly divided into six groups:control group,model group,cimetidine group(42.00 mg·kg^(−1),positive drug)and Biling Weitong Granules low-,middle-and high-dose group(0.79,1.58 and 3.16 g·kg^(−1)),eight mice in each group.The drugs were given by intragastric administration before the model was established,once a day for eight days,while the rats in the control group and model group were given 0.5%carboxymethylcellulose sodium(CMC-Na)solution by intragastric administration.Thirty minutes after the last administration,excluding rats in the control group,the rest of the rats were given 1 mL of absolute ethanol by intragastric administration to induce gastric ulcers.One hour later,all rats were sacrificed immediately.The gastric mucosal surface was unfolded to take pictures and the area of gastric ulcers was measured.HE staining of stomach sections were carried out to observe pathological changes.Interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),total superoxide dismutase(SOD),myeloperoxidase(MPO)and prostaglandin E_(2)(PGE_(2))were detected.Results Compared with the control group,the area of gastric ulcer and pathological score of gastric mucosa in the model group increased significantly(P<0.001),the levels of IL-1β(P<0.01)and TNF-α(P<0.05)in serum,MPO activity(P<0.001)in gastric tissue increased significantly,and the level of PGE_(2) in gastric tissue decreased significantly(P<0.01).Compared with the model group,the area of gastric ulcer and pathological score of gastric mucosa in each treatment group were significantly lower(P<0.05,0.01,0.001).Compared with the model group,the levels of serum IL-1βand TNF-αin Biling Weitong Granule group were significantly lower(P<0.05,0.001),the activity of MPO in gastric tissue was significantly lower(P<0.01,0.001),and the activity of serum SOD was significantly higher(P<0.05),the level of PGE_(2) in gastric tissue increased significantly(P<0.05).Conclusion Biling Weitong Granule can protect gastric ulcer model rats by inhibiting the secretion of inflammatory factors,alleviating oxidative stress and protecting gastric mucosa.