851例HIV/AIDS患者抗病毒治疗后病毒载量和CD4^(+)T淋巴细胞检测结果分析

Analysis of viral load and CD4^(+) T lymphocyte in 851 HIV/AIDS patients after receiving anti retro viral therapy

目的了解郑州市851例HIV/AIDS患者抗病毒治疗后免疫重建效果及病毒抑制情况,分析病毒载量与CD4^(+)T淋巴细胞的相关性。方法采集HIV/AIDS患者的外周血用于CD4^(+)T淋巴细胞的计数,分离血浆后采用荧光探针法检测病毒载量(VL)。应用SPSS 22.0对数据进行统计分析。结果抗病毒治疗后,58.99%患者CD4^(+)T淋巴细胞≥350个/μL,病毒抑制率(VL<1000)达96.94%,不同特征的HIV/AIDS患者CD4^(+)T淋巴细胞构成比和病毒抑制情况比较,差别无统计学意义(P>0.05)。不同病毒含量的HIV/AIDS患者CD4^(+)T细胞计数构成差异有统计学意义(χ^(2)=159.67,P<0.01)。Spearman检验结果显示,VL与CD4^(+)T淋巴细胞计数呈负相关(r=-0.129,P<0.01)。结论积极接受抗病毒治疗的HIV/AIDS患者,病毒抑制和免疫重建情况良好。联合应用外周血CD4^(+)T淋巴细胞检测和病毒载量检测能够较好指导抗病毒治疗效果。

Objective To investigate the immune system reconstitution effect and virus suppression of 851 HIV/AIDS patients after anti-viral therapy in Zhengzhou city,and to analyze the correlation between viral load and CD4^(+) T lymphocyte.Methods The peripheral blood of HIV/AIDS patients was collected for the count of CD4^(+) T lymphocyte.After the plasma was separated,the viral load(VL) was detected by the fluorescence probe method.SPSS 22.0 was used to analyze the collected data.Results After antiviral treatment,58.99% patients had CD4^(+) T lymphocyte≥350 cells/μL and the virus suppression rate(VL<1 000) reached 96.94%.The difference was not statistically significant(P>0.05)by comparing the ratio of CD4^(+) T lymphocyte and virus suppression in HIV/AIDS patients who have different characteristics.There was a statistically significant difference in the CD4^(+)T cell counts of HIV/AIDS patients with different virus content(χ^(2)=159.67,P<0.01).Spearman test results showed that VL was negatively correlated with CD4^(+) T lymphocyte count(r=-0.129,P<0.01).Conclusion HIV/AIDS patients who received actively antiviral therapy showed good viral suppression and immune system reconstitution.The combined application of peripheral blood CD4^(+) T lymphocyte detection and viral load detection can better guide the effect of antiviral treatment.