长链非编码RNA SNHG12靶向miR-199a对肿瘤细胞增殖、侵袭和转移的影响

Effects of long noncoding RNA SNHG12 targeting microRNA-199a on tumor cell proliferation,invasion and metastasis

目的探讨长链非编码RNA(lncRNA) SNHG12靶向微小RNA(miRNA, miR)-199a对肿瘤细胞增殖、侵袭和转移的影响。方法选取新乡医学院第一附属医院2017年9月到2021年9月手术摘除的123例宫颈癌组织和癌旁组织作为研究对象。荧光定量聚合酶链反应(PCR)分析宫颈癌组织和癌旁组织lncRNA SNHG12和miR-199a表达水平;宫颈癌Hela细胞随机分为lncRNA对照组、SNHG12 KD组, miRNA对照组和miR-199a。细胞计数试剂盒(CCK-8)和体外移植瘤实验分析两组细胞增殖能力;Transwell分析细胞侵袭能力;体内实验分析细胞淋巴结转移数量;荧光素酶基因报告实验检测lncRNA SNHG12和miR-199a的关系。计量资料比较采用t检验。结果癌旁组织lncRNA SNHG12表达水平(1.25±0.19)明显低于宫颈癌组织lncRNA SNHG12表达水平(2.98±0.23), 癌旁组织miR-199a表达水平(1.49±021)明显高于宫颈癌组织miR-199a表达水平(0.46±0.14), 差异有统计学意义(t=4.001、3.891, P<0.05)。lncRNA对照组细胞48 h吸光度(A)值(1.95±0.24)明显高于SNHG12 KD组细胞48 hA值(1.33±0.17), miRNA对照组细胞48 hA值(2.18±0.25)明显高于miR-199a组细胞A值(1.44±0.27), 差异有统计学意义(t=3.891、4.019, P<0.05)。lncRNA对照组细胞成瘤体积[(794.54±88.09) mm3]明显高于SNHG12 KD组细胞成瘤体积[(372.43±34.76) mm3], 差异有统计学意义(t=5.309, P<0.05)。lncRNA对照组细胞侵袭数量[(109.43±16.98)个]明显高于SNHG12 KD组细胞侵袭数量[(69.44±7.09)个], 差异有统计学意义(t=8.129, P<0.05)。lncRNA对照组细胞淋巴结转移数量[(12.43±3.09)个]明显高于SNHG12 KD组细胞成瘤体积[(5.09±2.98)个], 差异有统计学意义(t=2.891, P<0.05)。miR-199a是lncRNA SNHG12是靶点。lncRNA对照组细胞miR-199a表达水平(1.67±0.23)明显低于SNHG12 KD组细胞miR-199a表达水平(3.91±0.31), 差异有统计学意义(t=4.190, P<0.05)。结论 lncRNA SNHG12在宫颈癌组织中呈高表达, 通过与miR-199a吸附结合, 参与宫颈癌细胞的增殖、侵袭和转移等过程。

Objective To investigate the effects of long noncoding RNA(lncRNA)SNHG12 targeting microRNA(miR)-199a on tumor cell proliferation,invasion and metastasis.Methods Totally,123 cervical cancer tissues and adjacent tissues removed in our hospital from September 2017 to September 2021 were selected as the research objects.The expression levels of lncRNA SNHG12 and miR-199a in cervical cancer tissues and adjacent tissues were analyzed by fluorescence quantitative polymerase chain reaction(PCR).HeLa cells of cervical cancer were randomly divided into lncRNA control group,SNHG12 KD group,miRNA control group and miR-199a group.The cell proliferation ability of the two groups was analyzed by cell counting kit-8(CCK-8)and tumor transplantation experiment in vitro.Cell invasion ability was analyzed by Transwell.The number of cell lymph node metastasis was analyzed in vivo.Luciferase gene reporter assay was used to detect the relationship between lncRNA SNHG12 and miR-199a.The measurement data were compared by t-test.Results The expression level of lncRNA SNHG12 in adjacent tissues(1.25±0.19)was significantly lower than that in cervical cancer tissues(2.98±0.23).The expression level of miR-199a in adjacent tissues(1.49±021)was significantly higher than that in cervical cancer tissues(0.46±0.14,t=4.001,3.891,P<0.05).The 48 h absorbance(A)value(1.95±0.24)in lncRNA control group was significantly higher than that in SNHG12 KD group(1.33±0.17),and the 48 h A value(2.18±0.25)in miRNA control group was significantly higher than that in miR-199a group(1.44±0.27,t=3.891,4.019,P<0.05).The tumor forming volume of lncRNA control group[(794.54±88.09)mm3]was significantly larger than that of SNHG12 KD group[(372.43±34.76)mm3,t=5.309,P<0.05].The number of invasive cells in lncRNA control group[(109.43±16.98)]was significantly greater than that in SNHG12 KD group[(69.44±7.09),t=8.129,P<0.05].The number of lymph node metastases in lncRNA control group(12.43±3.09)was significantly greater than that in SNHG12 KD group(5.09±2.98,t=2.891,P<0.05).The expression level of miR-199a in lncRNA control group(1.67±0.23)was significantly lower than that in SNHG12 KD group(3.91±0.31,t=4.190,P<0.05).Conclusion lncRNA SNHG12 is over-expressed in cervical cancer.It participates in the proliferation,invasion and metastasis of cervical cancer cells by binding with miR-199a.