基于网络药理学探讨昆明山海棠治疗膜性肾病的作用机制

Mechanism of Tripterygium Hypoglaucum in the Treatment of Membranous Nephropathy Based on Network Pharmacology

目的:通过网络药理学探讨中药昆明山海棠治疗膜性肾病的潜在靶点及作用机制。方法:通过BATMAN-TCM筛选昆明山海棠的有效成分和靶点,通过GeneCards、人类孟德尔遗传综合数据库、治疗靶点数据库、DrugBank和GAD等数据库查找膜性肾病的相关靶点。利用Cytoscape软件分析昆明山海棠治疗膜性肾病的作用靶点与有效成分之间的关系网络,通过DAVID数据库分析昆明山海棠治疗膜性肾病的有效靶点的生物学功能和作用机制。结果:根据口服利用度和药物相似性原则筛选出昆明山海棠有效成分15个,筛选出的有效成分作用靶点78个,有关膜性肾病靶点3191个。通过基因本体功能富集分析,得到昆明山海棠干预膜性肾病的作用靶点的生物学功能,如类固醇结合性、核受体活性和直接配体调控特异性DNA序列结合性等。通过京都基因与基因组百科全书通路富集分析,筛选出昆明山海棠治疗膜性肾病的信号通路,如雷帕霉素靶蛋白信号通路等。结论:昆明山海棠治疗膜性肾病主要涉及类固醇结合性等多重生物学特性,干预雷帕霉素靶蛋白信号通路等多条信号通路。

OBJECTIVE:To probe into the potential targets and mechanism of tripterygium hypoglaucum in the treatment of membranous nephropathy based on network pharmacology.METHODS:Effective components and targets of tripterygium hypoglaucum were screened by using BATMAN-TCM.Related targets of membranous nephropathy were identified by GeneCards,Human Mendelian Genetic Comprehensive database,therapeutic target database,DrugBank and GAD.Cytoscape software was used to analyze the correlation between the target and active components of tripterygium hypoglaucum in the treatment of membranous nephropathy.Biological function and mechanism of effective target of tripterygium hypoglaucum for membranous nephropathy were analyzed by DAVID database.RESULTS:According to the principles of oral availability and drug similarity,15 active components of tripterygium hypoglaucum were screened out,and 78 active targets were screened out,and 3191 targets for MN were extracted from databases.Through gene enrichment analysis,the biological function of the targets of tripterygium hypoglaucum intervention in membranous nephropathy were obtained,such as steroid binding,nuclear receptor activity and direct ligand-regulated specific DNA sequence binding.Through pathway enrichment analysis of Kyoto Encyclopedia of Genes and Genomes,the signal pathway of membranous nephropathy treatment by tripterygium hypoglaucum was screened out,such as target rapamycin signaling pathway.CONCLUSIONS:The treatment of membranous nephropathy by tripterygium hypoglaucum mainly involves multiple biological properties such as steroid binding properties and intervention with multiple signaling pathways such as rapamycin target protein signaling pathway.