Sonic hedgehog诱导人脐带间充质干细胞分化过程中成骨相关miRNA表达差异的筛选

Identification of the different expression of osteogenic-related miRNAs in hUCMSCs induced by sonic hedgehog

目的:检测并分析人脐带间充质干细胞(hUCMSCs)成骨分化过程中的成骨特征性miRNA表达谱,探讨其可能的靶基因和临床意义。方法:对Shh诱导hUCMSCs成骨分化过程中表达的miRNA进行高通量测序分析,检测其表达量的变化,并验证成骨相关miRNA的表达差异。结果:通过miRNA高通量测序和RT-qPCR验证,预测miRNA-342-3p可能作为关键因子,通过靶向Sufu调控Shh信号通路,进而调控hUCMSCs的成骨分化。结论:筛选出新的调控因子miRNA-342-3p作为Shh信号通路的靶分子,对成骨分化过程进行精准调控,为种植义齿骨组织工程提供新的治疗思路。

Objective:To detect and analyze the osteogenic characteristic microRNA expression profile during the osteogenic differentiation of human umbilical cord mesenchymal stem cells(hUCMSCs),for exploring the potential target genes and clinical significance.Methods:Sequencing analysis performed on the miRNAs expressed in hUCMSCs which induced by sonic hedgehog.Osteogenic-related miRNAs with significant changes were identified.Results:As a key factor, miRNA-342-3 p may regulate the osteogenesis process through shh signaling pathway from the results of miRNA sequencing and RT-qPCR.Moreover, Sufu may be a targeting factor of miRNA-342-3 p by miRBase predicts.Conclusion:This study has screened out a new regulatory factor for shh signaling pathway.As the target molecule of Sufu, miRNA-342-3 p could be used to accurately regulate the bone remodeling process during implant-related bone tissue engineering.