靶向CD47的免疫治疗对口腔-肠道菌群影响的实验初探

A Preliminary Study on the Effect of CD47-Targeted Immunotherapy on Oral-Gut Microbiota

目的比较靶向CD47的免疫治疗对于免疫健全小鼠口腔-肠道菌群的影响。方法构建免疫健全小鼠的结肠癌腹腔转移模型,经腹腔实验组给予抗CD47单克隆抗体或对照组给予PBS溶液进行治疗,用小动物活体成像技术记录肿瘤生长,采用16S rRNA基因测序技术进行口腔-肠道微生物群落组成及多样性分析。结果抗CD47单克隆抗体治疗组小鼠口腔微生物Alpha多样性降低,差异有统计学意义(P<0.05);肠道微生物Alpha多样性则无显著改变;差异物种分析显示,接受治疗小鼠口腔葡萄球菌属(Staphylococcus)、Jeotgalicoccus、球菌属(Sporosarcina)较对照组丰度显著降低,肠道菌群拟杆菌属(Bacteroides)在接受靶向CD47免疫治疗组丰度显著增高。结论靶向CD47的免疫治疗对小鼠口腔菌群多样性有着较大的影响,而对肠道菌群物种多样性无显著影响。

Objective To study the effects of CD47-targted immunotherapy on the oral-gut microbiota of immune-competent mice.Methods A peritoneal metastatic colon cancer model was constructed in immune-competent mice.Anti-CD47 monoclonal antibody was intraperitoneally administered to the mice in the treatment group,while PBS was administered to mice in the control group.Tumor growth was documented with small animal live imaging technology.16S rRNA sequencing technology was used to analyze the composition and diversity of oral-gut microbiota.Results The alpha diversity of oral microbes in the anti-CD47 monoclonal antibody treatment group decreased,and the difference was statistically significant.There was no significant change in the alpha diversity of gut microbes.Differential species analysis showed significantly decreased abundance of Staphylococcus,Jeotgalicoccus,and Sporosarcina in the oral microbiota of mice in the treatment group compared to that of mice in the control group.The abundance of Bacteroides in the gut microbiota was significantly higher in the treatment group.Conclusion CD47-targted immunotherapy has a rather significant impact on the diversity of oral microbiota in mice,but does not have significant impact on the species diversity of gut microbiota.