基于ITS序列的DNA条形码技术鉴定罂粟科种子

Identification of Papaveraceae seeds by DNA Barcoding Based on ITS Sequence

采用基于ITS4/ITS5序列的DNA条形码分子生物学技术,结合形态学和地理分布信息,对满洲里口岸进境俄罗斯粮食中检出的2种未知种子进行检疫鉴定。结果表明,2种未知种子分别为红花疆罂粟[Roemeria refracta(Stev.)DC.]和长荚罂粟(Papaver dubium L.)。本方法具有快速、简便、准确的特点,有效弥补了传统形态学分类鉴定的不足,适用于进境产品中植物果实或种子的检疫鉴定。

Using DNA barcoding molecular biology technology based on ITS4/ITS5 sequence,combined with morphological and geographical distribution analysis,two unknown seeds detected in Russian grain at Manzhouli Port were quarantined and identified.The results showed that the two unknown seeds were Roemeria refracta(Stev.)DC.and Papaver dubium L.This method is fast,simple and accurate,which effectively makes up for the shortcomings of traditional morphological classification and identification,and is suitable for the quarantine and identification of plant fruits or seeds in imported products.