基于iTRAQ技术分析镉胁迫下泽兰实蝇雄性附腺差异蛋白的表达

Differential Proteomic Analysis of the Male Accessory Gland of Procecidochares utilis Stone under Cadmium Stress Based on iTRAQ Technique

【目的】明确镉污染对泽兰实蝇(Procecidochares utilis Stone)雄性附腺蛋白质造成的影响。【方法】利用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantification,iTRAQ)技术结合MS/MS对染镉与未染镉的泽兰实蝇雄性附腺蛋白质进行分析与鉴定。【结果】共鉴定出3 661个蛋白质,具有定量信息差异的蛋白质58个,其中上调的蛋白质38个,下调的蛋白质20个。GO和KEGG注释分析和富集分析结果显示:这些蛋白质具有催化、代谢和转运等功能,表明染镉后泽兰实蝇雄性附腺中多种功能的蛋白质发生了变化。【结论】本研究明确了镉污染可改变泽兰实蝇雄性附腺蛋白质组,并鉴定了镉胁迫后泽兰实蝇雄性附腺的差异蛋白。

[Purpose]To clarify the effects of cadmium pollution on the proteome of the male accessory gland of Procecidochares utilis Stone. [Method]Isobaric tags for relative and absolute quantification(iTRAQ) technique and MS/MS were used to analyze and identify the proteome of the male accessory gland of P. utilis with and without cadmium. [Results]A total of 3 661 proteins were identified, of which 58 had quantitative information differences, 38 were up-regulated and 20were down-regulated. GO and KEGG annotation analysis and enrichment analysis were carried out on the identified differential proteins, and it was found that these proteins had catalytic, metabolic and transport functions. The results showed that the functional proteins in the male accessory glands of P.utilis were changed after cadmium-stained. [Conclusion]This study clarified the changes in the proteome of the male accessory glands of P. utilis caused by cadmium pollution, and identified the differential proteins of the male appendages of P. utilis after cadmium stress.