摘要
芸薹属蔬菜种质资源丰富,是中国蔬菜产业的重要组成部分。游离小孢子培养是创造单倍体和双单倍体的重要途径,对提高芸薹属蔬菜的育种效率具有重要意义。为了研究芸薹属蔬菜游离小孢子的高效培养体系,归纳了植物材料、预处理方式、培养基成分、培养密度、高温热激、植株再生、植株的倍性鉴定与加倍等多个因素对小孢子培养的影响,提出了可通过优化培养体系、遗传转化和共培养等方式提高芸薹属蔬菜小孢子培养效率的观点。
Brassica vegetable is rich in germplasm resources and has very important role in China’s vegetable industry.Isolated microspore culture is a considerable way to create haploid and double haploid,and is significant to improve the breeding efficiency of Brassica vegetables.In order to study the efficient culture system of isolated microspore of Brassica vegetables,the effects of plant materials,pretreatment methods,medium composition,culture density,heat shock,plantlet regeneration,ploidy identification and diploid inducement on microspore culture were summarized.It is proposed that the efficiency of isolated microspore culture of Brassica vegetables could be improved by the optimization of cultivation system,genetic transformation and co-culture treatment.
作者
伍健缤
陈坤豪
陈木溪
陈长明
WU Jianbin;CHEN Kunhao;CHEN Muxi;CHEN Changming(College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Guangdong Helinong Seeds,CO.,LTD.,Shantou 515800,Guangdong,China;Guangdong Helinong Agricultural Research Institute,CO.,LTD.,Shantou 515800,Guangdong,China)
出处
《农学学报》
2022年第3期44-49,共6页
Journal of Agriculture
基金
广东省基础与应用基础研究基金“转录因子BocMYB28调控芥蓝萝卜硫苷生物合成的分子网络解析”(2020A1515011396)
广州市农业农村科技特派员项目“菜心新品种选育技术研发、应用和推广”(GZKTP202020)
广州市科技计划项目“菜心品质性状SNP分子标记及优质新品种选育研究”(202002020007)
广东省重点领域研发计划“优质多抗菜心芥蓝新品种选育”(2018B020202010)。
关键词
芸薹属蔬菜
游离小孢子
胚胎发生
植株再生
双单倍体
育种
Brassica vegetables
isolated microspores
embryogenesis
plantlet regeneration
double haploid
breeding
