摘要
为探索建立紫椴组织培养快速繁育体系,以紫椴种子为材料,探讨基本培养基、植物生长调节剂浓度及配比对紫椴组培快繁效果的影响。结果表明,增殖培养基以1/2MS培养基最适宜,添加0.05 mg/L 6-BA和0.03 mg/L IBA后,增殖系数可达13.5;MS培养基最适宜做壮苗培养基和生根培养基,最佳壮苗培养基为MS+0.1 mg/L6-BA+0.1 mg/L IBA+0.03 mg/LGA_(3),平均苗高达5.15 cm;最佳生根培养基为MS+1.0 mg/L6-BA+0.05 mg/L NAA,生根率达93.3%,生根数达5.7根。
To explore the establishment of tissue culture and rapid propagation system of Tilia amurensis,the effects of basic medium,plant growth regulator concentration and ratio on tissue culture and rapid propagation of T.amurensis were studied.The results show that 1/2MS medium is the most suitable medium for proliferation,and the proliferation coefficient could reach 13.5 when 0.05 mg/L 6-BA and 0.03 mg/L IBA are added.MS medium is the most suitable medium for strong seedling and rooting,the best medium for strong seedling is MS+0.1 mg/L 6-BA+0.1 mg/L IBA+0.03 mg/L GA_(3),with an average seedling height of 5.15 cm.The best rooting medium is MS+1.0 mg/L 6-BA+0.05 mg/L NAA,the rooting rate is 93.3%,and the number of roots is 5.7.
作者
李庆华
王因花
孔雨光
姚俊修
鲁仪增
燕丽萍
吴德军
任飞
葛文华
LI Qinghua;WANG Yinhua;KONG Yuguang;YAO Junxiu;LU Yizeng;YAN Liping;WU Dejun;REN Fei;GE Wenhua(Shandong Academy of Forestry Sciences/Shandong Key Laboratory of Forest Tree Genetic Improvement,Jinan 250014,Shandong,China;Shandong Territorial and Spatial Planning Institute,Jinan 250014,Shandong,China;Shandong Forest Germplasm Resources Center,Jinan 250014,Shandong,China;Dashawa Forest Farm of Rizhao,Rizhao 276825,Shandong,China)
出处
《农学学报》
2022年第1期60-64,共5页
Journal of Agriculture
基金
山东省重点研发计划项目“椴树高效繁育及产业化升级关键技术研究”(2017GNC10112)
山东省农业重大应用技术创新项目“泰山椴等珍稀树种高效培育技术集成与示范”(鲁财农指[2016]36号)
山东省农业良种工程项目“珍贵用材树种种质资源收集保存与精准鉴定”(2019LZGC01804)。
关键词
紫椴
组织培养
壮苗培养基
生根培养基
无性繁殖
快繁技术
Tilia amurensis
Tissue Culture
Medium for Strong Seedling
Medium for Rooting
Vegetative Propagation
Rapid Propagation
