香樟及近缘种的亲缘关系分析

Genetic relationship analysis of Cinnamomum camphora and its related species

基于全基因组重测序技术开发的SNP标记,对香樟及近缘种的亲缘关系进行分析,结果表明,通过对16个香樟无性系和2个近缘种进行重测序分析,共获得209 Gb原始数据,平均有效测序深度16×.共获得约209308831个高质量SNP,纯合SNP共165814908个,杂合SNP共43493923个.由系统进化树可知11份芳樟无性系聚在一起,3份芳樟无性系与油樟聚在一起,然后与龙脑樟聚在一起,沉水樟和肉桂先被分离出来;通过主成分分析将16份香樟无性系分为3类,分别为芳樟类群、龙脑樟和油樟类群;芳樟、龙脑樟和油樟精油成分存在差异,为不同的化学利用类型.

Based on single nucleotide polymorphism(SNP)markers developed from whole genome resequencing technology,the phylogenetic relationship of 16 Cinnamomum camphora clones and 2 related species were analyzed to provide a theoretical basis for the origin,evolution and systematic classification of C.camphora.A total of 209 Gb clean data were generated,with an average sequencing depth of 16×.A total of 209308831 high-quality SNPs were identified,including 165814908 homozygous and 43493923 heterozygous SNPs.Phylogenetic tree demonstrated that the divergence of C.micranthum and C.cassia occurred first;then C.longipaniculatum was separated from the 14 C.camphora clones,which was further divided into 2 clusters,11 grouped together and the other 3 clustered with C.camphora chvar.Borneol.Principal component analysis confirmed that the 16 C.camphora clones were divided into 3 categories of C.camphora var.inaloolifera Fujita.group,C.longipaniculatum group and C.camphora chvar.Borneol.Due to variations in the composition of essential oil,the 3 groups of Cinnamomum had different chemical utilizations.