脊尾白虾EcERR基因的克隆与表达分析

Cloning and Expression Analysis of EcERR in Exopalaemon carinicauda

雌激素相关受体(estrogen-related receptor,ERR)是一种真核转录因子,与雌激素的效应有密切关系,参与生物体的能量代谢、细胞增殖分化和性腺发育等生理过程。为了解ERR基因在脊尾白虾(Exopalaemon carinicauda)卵巢发育中的作用,采用cDNA末端快速扩增技术(RACE)克隆获得脊尾白虾EcERR基因的c DNA序列,该基因全长为2025 bp,包括5′非编码区241 bp,3′非编码区410bp,开放阅读框1374bp,编码457个氨基酸。组织表达分析结果显示,EcERR在脊尾白虾各组织中均有表达,卵巢组织的表达量最高(P<0.05);EcERR在卵裂期的表达量显著高于胚胎及幼体发育时期(P<0.05);EcERR在卵巢发育过程中呈先降低后升高的趋势,卵巢发育Ⅰ期的表达量最高;5-羟色胺浸泡后,实验组EcERR的表达量显著高于对照组(P<0.05),同时,实验组卵巢发育关键基因卵黄蛋白原基因的表达量显著高于对照组(P<0.05)。研究表明,EcERR基因参与了脊尾白虾卵巢发育的调控,在脊尾白虾卵巢发育的早期发挥关键作用。

Estrogen-related receptor(ERR)is a eukaryotic transcription factor that is closely related to the effect of estrogen and is involved in the regulation of energy metabolism,cell proliferation and differentiation,gonadal development,and other physiological processes.To understand the role of estrogen-related receptors in the ovarian development of Exopalaemon carinicauda,the EcERR was cloned using RACE technology.The gene is 2025 bp long,including an open reading frame of 1374 bp,a 5′untranslated region of 241 bp,and a 3′untranslated region of 410 bp,which encodes 457 amino acids.Tissue expression analysis showed that EcERR was expressed in many E.carinicauda tissues,with the highest expression level in ovarian tissue.The expression level of EcERR in the cleavage stage was significantly higher than in that of other embryo and larval development stages(P<0.05).The expression level of Ec ERR first decreased and then increased during ovarian development,with the highest expression level in StageⅠ.After 5-hydroxytryptamine invaded,the expression levels of vitellogenin and EcERR in the experimental group were significantly higher than in the control group(P<0.05).The results showed that EcERR is involved in the regulation of E.carinicauda ovarian development and plays a key role in its early stages.