马氏珠母贝(Pinctada fucata)血细胞全长转录组测序与分析

Full-length Transcriptome Sequencing from Hemocytes of Pinctada fucata

[目的]开发马氏珠母贝(Pinctada fucata)基因组资源,挖掘功能基因。[方法]以马氏珠母贝血细胞为试材,利用单分子实时技术(single-molecule real time,SMRT)进行全长转录组测序,并对所获得unigenes进行功能注释和基因结构分析。[结果]共获得277064条全长非嵌合序列和82381个基因。经过比对nr、SwissProt、KEGG和KOG数据库进行注释和功能分类后,共得到59621个注释基因。同时,在8493条基因中共发现11219个SSR位点,其中以二核苷酸重复基元类型最高(50.9%)。生物信息学分析鉴定得到20013个lncRNA、2004个转录因子、10522个可变剪切分析位点。[结论]使用SMRT技术能够深入挖掘马氏珠母贝全长转录组数据,为进一步探讨马氏珠母贝功能基因的挖掘、免疫响应及遗传机制的研究提供可靠的基因组资源。

[Objective]To deep analysis of the genomic resources of the pearl oyster(Pinctada fucata).[Method]The single-molecule real time technology(SMRT)was performed to sequence the full-length transcriptome from hemocytes,and functional annotation and the structure of the unigenes were analyzed.[Result]The results showed that a total of 277064 full-length non-chimeric sequences were generated,and 82381 unigenes were finally obtained.By comparing the nr,SwissProt,KEGG and KOG databases for annotation and functional classification,a total of 59621 unigenes were annotated.GO function annotation showed that most genes were mainly enriched in cellular process,metabolic process and single biological process of biological process.Annotated genes in cell component were mainly enriched in cell and cell part,while in molecular function,they were mainly concentrated in binding and catalytic activity.Meanwhile,11219 SSR sites were identified in 8493 unigenes,among which the proportion of dinucleotide repeat motif was the highest(50.9%).Additionally,the results also identified 20013 lncRNAs,2004 transcription factors and 10522 alternative splicing sites.[Conclusion]These results may improve knowledges on transcriptome data of P.fucata using SMRT,and provide reliable genomic resources for further exploring the development of functional genes,growth traits related gene markers,immune response and genetic mechanisms.