基于SSR分子标记的早熟棉种质资源遗传多样性分析

Genetic diversity analysis of early maturing cotton germplasm resources base on SSR molecular markers

【目的】明确内蒙古自治区农牧业科学院棉花研究中心早熟棉种质资源的亲缘关系,为杂交组合的选配及定向育种提供亲本材料。【方法】采用SSR分子标记(微卫星DNA),利用31对SSR引物对44份早熟棉种质资源的遗传多样性进行分析鉴定。【结果】31对引物共扩增出多态性条带72条,平均每对引物扩增出2.25条。聚类分析结果显示,各材料间的遗传相似系数为0.35~0.80,平均为0.58。其中,酒棉17号与中716的遗传相似系数最小,为0.35;新陆早41号与中361、中夏05与辽棉15号的遗传相似系数最大,均为0.80。【结论】31对SSR引物在供试材料间的多态性较好,44份早熟棉材料遗传变异类型丰富,初步揭示了早熟棉种质资源间的遗传背景差异及亲缘关系。

【Objective】To clarify the genetic relationship of the early maturing cotton germplasm resources in the Cotton Research Center of the Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,in order to provide parental materials for hybrid combination selection and directional breeding.【Methods】Using SSR molecular markers(microsatellite DNA),31 pairs of SSR primers were used to investigate and identify the genetic diversity of 44 early maturing cotton germplasm resources.【Results】31 pairs of primers amplified a total of 72 polymorphic bands,with an average of 2.25 bands per pair of primers.The genetic similarity coefficient among the materials ranged from 0.35 to 0.80,with an average of 0.58,according to the results of cluster analysis.The genetic similarity coefficient between Jiumian No.17 and Zhong 716 was the lowest,at 0.35.The highest genetic similarity coefficient were 0.80 for Xinluzao No.41 and Zhong 361,Zhongxia 05 and Liaomian No.15.【Conclusion】The 31 pairs of SSR primers were effective in amplifying the polymorphisms among the materials studied,and the genetic diversity types of 44 early maturing cotton materials were abundant,revealed the genetic background differences and genetic relationship among early maturing cotton germplasm resources.