摘要
目的:研究草果甲醇溶出物对α-葡萄糖苷酶的抑制作用及对小鼠高血糖的调节作用。方法:采用4-硝基酚-α-D-吡喃葡萄糖苷法(PNPG),以阿卡波糖为阳性对照,进行α-葡萄糖苷酶体外抑制试验,评估草果甲醇溶出物的α-葡萄糖苷酶抑制率。用高脂饲料喂养小鼠构建异常血糖小鼠模型,在高脂饲料基础上,分别添加100、200 mg/kg的草果甲醇溶出物干预6周,评估草果甲醇溶出物对小鼠空腹血糖、葡萄糖耐量以及胰岛素抵抗的影响。结果:当草果甲醇溶出物溶液浓度为500 mg/L时,对α-葡萄糖苷酶的抑制率达到63.72%。草果甲醇溶出物对α-葡萄糖苷酶的半数抑制浓度(IC_(50))为0.145 mg/mL。在经灌胃低、高剂量组的草果甲醇溶出物干预2周后,小鼠空腹血糖开始降低并维持稳定,草果低、高剂量组葡萄糖耐量曲线下面积(AUC)在干预6周后均低于模型组(P<0.05)。结论:草果甲醇溶出物可明显抑制α-葡萄糖苷酶活性,并能明显改善小鼠葡萄糖耐量水平。
Objective:To investigateα-glucosidase inhibitory and high blood glucose regulation of methanol extracts from Amomum tsao-ko in aged mice.Methods:Using 4-N-trophenyl-α-D-glu-copyranoside(PNPG)method with acarbose as positive control,α-glucosidase inhibition test in vitro was carried out to evaluate α-glucosidase inhibition rate of methanol extract from Amomum tsao-ko.The mouse model of abnormal blood glucose was established by feeding mice with high-fat diet.On the basis of high-fat diet,100 and 200 mg/kg of methanol extracts from Amomum tsao-ko were added for 6 weeks to evaluate the effects of methanol extracts from Amomum tsao-ko on fasting blood glucose,glucose tolerance and insulin resistance in mice.Results:When the concentration of methanol extracts solution was 500 mg/L,the inhibition rate of α-glucosidase reached 63.72%.Half inhibitory concentration(IC_(50))of methanol extract from Amomum tsao-ko on α-glucosidase was 0.145 mg/mL.The fasting blood glucose of mice in the herb group began to decrease and maintain stability after 2 weeks of intervention by intragastric administration of low and high dose methanol extracts of Amomum tsao-ko groups.The area under the curve(AUC)values of glucose tolerance curve in low and high dose methanol extracts of Amomum tsao-ko groups after 6 weeks intervention were lower than that of model group(P<0.05).Conclusion:Methanol extracts of Amomum tsao-ko could obviously inhibit α-glucosidase activity and improve glucose tolerance in mice.
作者
解立斌
陈佳
剧慧栋
胡连霞
韩爱云
俞龙泉
XIE Libin;CHEN Jia;JU Huidong;HU Lianxia;HAN Aiyun;YU Longquan(School of Chemical Engineering,Shijiazhuang University,Shijiazhuang 050035,China)
出处
《食品工业科技》
CAS
北大核心
2022年第8期382-388,共7页
Science and Technology of Food Industry
基金
河北省高等学校科学技术研究项目(QN2020525)
石家庄学院博士科研启动基金(21BS004)。
关键词
草果
甲醇溶出物
Α-葡萄糖苷酶
血糖
Amomum tsao-ko
methanol extracts
α-glucosidase
blood glucose