盐酸莱克多巴胺-D_(6)新的合成方法研究与结构表征

Novel Synthetic Method and Characterization of Ractopamine-D_(6) Hydrochloride

稳定同位素内标试剂与同位素稀释质谱法相结合的检测技术,为快速准确地检测莱克多巴胺的含量提供了可能。以4-(4-甲氧苯基)-2-丁酮为起始原料,经氢-氘交换、还原胺化、脱保护基反应,合成稳定同位素氘标记的盐酸莱克多巴胺。该合成路线具有路线短、操作简便、原料廉价易得等优点。合成产物经核磁共振氢谱(^(1)HNMR)和质谱(ESI-MS)表征确认,氘同位素丰度为97.7 atom%D,可作为质谱内标试剂应用于兽残检测领域。

Stable isotope internal standard reagent combined with isotope dilution mass spectrometry can offer a feasible method with accuracy and precision for the detection of ractopamine.A deuterium-labeled ractopamine hydrochloride was synthesized from 4-(4-methoxyphenyl)-2-butanone via the steps of H-D isotope exchange,reduction amination,and deprotection.The novel synthetic route has the advantages of short route,easy operation,cheap,accessible starting materials,and high isotope abundance and so on.The deuterium-labeled product was confirmed by nuclear magnetic resonance(^(1)HNMR) and mass spectrometry(ESI-MS) characterization with more than 97 atom% D deuterium isotope abundance,which can be used as MS internal standards in the field of veterinary residues detection.