Cu^(2+)暴露下蛋白核小球藻的生理响应研究

Physiological response of Chlorella pyrenoidosa to Cu^(2+) exposure

在现代社会发展中解决重金属污染问题已刻不容缓,本试验研究了蛋白核小球藻(Chlorellapyrenoidosa)对重金属污染物铜离子(Cu^(2+))长期暴露下生理响应机制,为评价Cu^(2+)的生态健康风险提供理论依据。试验结果表明:不同浓度Cu^(2+)对C.pyrenoidosa表现出不同程度的促进或抑制效果,低浓度组(0.10 mg/L、0.50 mg/L)Cu^(2+)在短期内(2 d)可促进C.pyrenoidosa生长,使叶绿素和蛋白质含量迅速增加,随暴露时间延长(14 d)比对照组更快增殖至环境最大容纳量。中高浓度组(1 mg/L、2 mg/L)则与之相反,在2 d内生长受抑制,随着14 d的暴露,细胞产生耐受性后的叶绿素和蛋白质含量逐渐升高。

It is imperative to solve the problem of heavy metal pollution in the development of modern society,In this experiment,the physiological response mechanism of Chlorella pyrenoidosa to long-term exposure of heavy metal pollutants copper ions(Cu^(2+))was studied,which provided a theoretical basis for evaluating the ecological health risk of Cu^(2+).The test results showed that different concentration of Cu^(2+) showed different degrees of promoting or inhibiting effects on C.pyrenoidosa,and the low concentration group(0.10 mg/L,0.50 mg/L)Cu^(2+) could promote the growth of C.pyrenoidosa,The content of chlorophyll and protein increased rapidly,and then with the prolonged exposure time(14 d),it proliferated to the maximum environmental capacity faster than the con-trol group.On the contrary,in the middle and high concentration(1 mg/L,2 mg/L),growth were inhibited within 2 d,and with the exposure of 14 d,the contents of chlorophyll and protein gradually increased after the cells developed tolerance.