摘要
为探讨斑玉蕈(Hypsizygus marmoreus)交配型位点结构及其多态性,通过制备双核菌株H3、X3和B3的原生质体及再生获得其单核菌株,分别命名为H3-1、X3-1、B3-1,其他按顺序编号;H3-1、X3-1、B3-1分别与同一菌株的其他单核菌株两两配对,各选取1对亲和单核菌株,两两配对测定交配型,采用生物信息学分析比较基因组重测序后不同单核菌株的A和B交配型位点结构及其多态性。结果表明:双核菌株H3、X3、B3分别分离得到2、13、5对亲和单核菌株,各选取的1对亲和单核菌株两两配对,确定6个单核菌株H3-1、H3-8、X3-1、X3-13、B3-1、B3-2的交配型分别为A1B1、A2B2、A1B3、A3B1、A4B1、A5B4;6个单核菌株的A交配型位点两翼紧邻MIP和B-fg保守基因,且HD2.2结构域共线性良好,单核菌株H3-8和B3-1的A交配型位点结构完全相同,6个单核菌株共包括5种A交配型位点结构,表明A交配型位点具有丰富的多态性;单核菌株X3-1包含5个信息素受体,单核菌株X3-13、H3-1、B3-2均包含7个信息素受体,单核菌株H3-8、B3-1均包含6个信息素受体,6个单核菌株B交配型位点共包括4种,单核菌株H3-1、X3-13、B3-1、H3-8、B3-2均包含1个信息素前体,单核菌株X3-1包含2个信息素前体,B交配型位点结构上下游基因的共线性较差,表明B交配型位点具有丰富的多态性。
The genetic structure and polymorphism of mating-type loci in Hypsizygus marmoreus were studied through sequencing six monokaryotic strains segregated from three dikaryotic H. marmoreus strains, H3, X3 and B3, respectively. The six monokaryotic strains were H3-1, H3-8, X3-1, X3-13, B3-1 and B3-2. Each pair of monokaryotic strains derived from the same dikaryotic strain were compatible and contained different mating types. The results showed that there were five kinds of A mating type locus structures and four kinds of B mating type locus structures in the six monokaryotic strains, respectively. The mating types of H3-1, H3-8, X3-1, X3-13, B3-1 and B3-2 were A1B1, A2B2, A1B3, A3B1, A4B1 and A5B4, respectively. H3-8 and X3-1 had the same A mating type locus structure. All the A mating-type loci were flanked by conserved MIP and B-fg at the two ends respectively, and had a tight collinearity in HD2.2 domain. For B mating type loci, there were five pheromone receptors in X3-1, six pheromone receptors in H3-8 and B3-1, and seven pheromone receptors in X3-13, H3-1 and B3-2. There was one pheromone precursor in H3-1, X3-13, B3-1, H3-8 and B3-2. X3-1 contained two pheromone precursors. The collinearity between genes upstream and downstream of B mating type loci was poor, suggesting a rich genetic diversity.
作者
马丽娟
李晓玲
鲍大鹏
尚俊军
周陈力
杨瑞恒
MA Lijuan;LI Xiaoling;BAO Dapeng;SHANG Junjun;ZHOU Chenli;YANG Ruiheng(School of Chemistry and Life Science,Changchun University of Technology,Changchun 130012,Jilin,China;School of Food and Pharmaceutical Engineering,Wuzhou University,Wuzhou 543002,Guangxi,China;Institute of Edible Fungi,Shanghai Academy of Agriculture Science,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agriculture Genetics and Breeding of Shanghai,Shanghai 201403,China)
出处
《食用菌学报》
CSCD
北大核心
2022年第2期23-30,共8页
Acta Edulis Fungi
基金
上海市现代农业产业技术体系[沪农科产字(2021)第9号]。
关键词
斑玉蕈
原生质体
交配型位点
亲和
Hypsizygus marmoreus
protoplast
mating-type locus
compatibility
