摘要
通过测定ITS序列对金耳(Naematelia aurantialba)子实体及栽培料中不同部位的真菌物种进行鉴定,结果表明,金耳子实体是由金耳菌丝和毛韧革菌(Sterem hirsutum)菌丝组成的复合结构,两者相互混合交织构成子实体,栽培料中只含毛韧革菌。在菌包底部注射^(15)N-铵态硝酸铵,利用稳定性同位素示踪技术对金耳子实体生长发育过程中的物质运输进行追踪。结果表明:在菌包中,^(15)N标记物被栽培料中的毛韧革菌菌丝吸收后运输至金耳子实体供金耳子实体生长发育。
Composition of fungal species in fruiting body and cultivation substrate of Naematelia aurantialba culture were identified by ITS sequencing. The results showed that the fruiting body samples were composites composed of both N. aurantialba and Sterem hirsutum, whereas the cultivation substrate samples only contained S. hirsutum. Using^(15)N-ammonium nitrate as the marker, nutrient transportation during the fruiting body development of N. aurantialba was tracked. The results showed that the^(15)N maker was initially absorbed by S. hirsutum and than transported to fruiting bodies of N. aurantialba.
作者
曹瑶
杨林雷
李荣春
冯发均
李梦杰
罗祥英
沈真辉
陆青青
CAO Yao;YANG Linlei;LI Rongchun;FENG Fajun;LI Mengjie;LUO Xiangying;SHEN Zhenhui;LU Qingqing(Yunnan Junshijie Biotechnology Ltd.,Kunming 650200,Yunnan,China;Institute of Edible Fungi,Yunnan Agricultural University,Kunming 650201,Yunnan,China)
出处
《食用菌学报》
CSCD
北大核心
2022年第2期48-53,共6页
Acta Edulis Fungi
基金
国家自然科学基金——云南联合项目(U1802231)。
关键词
金耳
ITS
稳定性同位素
物质运输
Naematelia aurantialba
ITS
stable isotope
nutrient transportation
