头针疗法对缺血性中风大鼠脑神经元的保护作用及对PI3K/AKt通路的影响

The protective effect of scalp acupuncture on cerebral neurons in rats with ischemic stroke and its influence on PI3K/AKt pathway

目的探讨头针疗法对缺血性中风大鼠脑神经元保护作用和对运动功能的作用及可能机制。方法用改良Longa线栓法制备缺血性中风大鼠模型,随机分为模型组、头针治疗组、抑制剂组、头针治疗+抑制剂组,另设假手术组。干预治疗结束后评价各组大鼠神经功能、行为学及运动功能;TTC染色法检测脑梗死面积;尼氏染色观察大鼠神经元形态;qPCR和Western Blotting分别检测大鼠缺血侧脑组织磷脂酰肌醇3激酶(PI3K)/丝氨酸-苏氨酸蛋白激酶(AKt)通路相关基因mRNA和蛋白表达水平。结果抑制剂组、模型组、头针治疗+抑制剂组和头针治疗组,神经功能损害及运动功能评分逐渐降低、逃避潜伏期逐渐缩短、目标象限停留时间逐渐延长、脑梗死面积比例逐渐降低、尼氏小体数量逐渐增多、PI3K、AKt mRNA和蛋白相对表达量逐渐升高(P<0.05),神经元形态逐渐改善。结论头针疗法对缺血性中风大鼠脑神经元具有保护作用,可增强其运动功能,作用机制可能与调控PI3K/AKt信号通路有关。

Objective To investigate scalp acupuncture’s protective effect on brain neurons and its effect on motor function in ischemic stroke rats and the possible mechanisms.Methods The rat model of ischemic stroke was prepared by the modified Longa suture method;the rats were randomly divided into model group,scalp acupuncture treatment group,inhibitor group,scalp acupuncture treatment+inhibitor group,and sham operation group.After a series of interventions,we evaluated the neurological,behavioral and motor functions of rats in each group after the treatment was over.TTC staining was used to detect cerebral infarction area.Neuron morphology was observed by Nissl staining.qPCR and Western blotting were used to detect the mRNA and protein expression levels of genes related to phosphatidyl inositol-3-kinase(PI3K)/serine-threonine protein kinase(AKt)pathway in the ischemic brain tissue of the rats.Results In the inhibitor group,the model group,the scalp acupuncture treatment group,and the scalp acupuncture treatment+inhibitor group,the neurological impairment and motor function score gradually decreased,the escape latency gradually shortened,the target quadrant residence time prolonged,the proportion of cerebral infarction area decreased,the number of Nissl bodies increased,the relative expressions of PI3K and AKt mRNA and protein increased(P<0.05),and the neuronal morphology improved.Conclusion Scalp acupuncture can protect cerebral neurons of rats with ischemic stroke and enhance their motor function.The mechanism may be related to the regulation of PI3K/AKt signaling pathway.